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Human Creatine kinase MT 1A protein, His tag (active)

Human Creatine kinase MT 1A protein, His tag (active). GTX66950-pro

Cat No. GTX66950-pro

Application

Functional Assay

Species

Human
Package
100 μg ($289)

APPLICATION

Application Note

Specific activity is > 50 units/mg, and is defined as the amount of enzyme that convert 1.0 μmole of phosphate from phosphocreatine to ADP per minute at pH 7.5 at 37ºC.

PROPERTIES

Form

Liquid

Buffer

20 mM Tris-HCl buffer (pH 8.0) with 0.15 M NaCl, 10% glycerol.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml

Region/Sequence

N-terminal His-Tag; Length: 403 a.a. Sequence: MGSSHHHHHH SSGLVPRGSH MGSHMASERR RLYPPSAEYP DLRKHNNCMA SHLTPAVYAR LCDKTTPTGW TLDQCIQTGV DNPGHPFIKT VGMVAGDEET YEVFADLFDP VIQERHNGYD PRTMKHTTDL DASKIRSGYF DERYVLSSRV RTGRSIRGLS LPPACTRAER REVERVVVDA LSGLKGDLAG RYYRLSEMTE AEQQQLIDDH FLFDKPVSPL LTAAGMARDW PDARGIWHNN EKSFLIWVNE EDHTRVISME KGGNMKRVFE RFCRGLKEVE RLIQERGWEF MWNERLGYIL TCPSNLGTGL RAGVHIKLPL LSKDSRFPKI LENLRLQKRG TGGVDTAATG GVFDISNLDR LGKSEVELVQ LVIDGVNYLI DCERRLERGQ DIRIPTPVIH TKH

Expression System

E. coli

Purity

> 95% by SDS-PAGE.

Conjugation

Unconjugated

Note

For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

creatine kinase, mitochondrial 1A , CKMT1 , U-MtCK , mia-CK

Background

Mitochondrial creatine (MtCK) kinase is responsible for the transfer of high energy phosphate from mitochondria to the cytosolic carrier, creatine. It belongs to the creatine kinase isoenzyme family. It exists as two isoenzymes, sarcomeric MtCK and ubiquitous MtCK, encoded by separate genes. Mitochondrial creatine kinase occurs in two different oligomeric forms: dimers and octamers, in contrast to the exclusively dimeric cytosolic creatine kinase isoenzymes. Many malignant cancers with poor prognosis have shown overexpression of ubiquitous mitochondrial creatine kinase; this may be related to high energy turnover and failure to eliminate cancer cells via apoptosis. Ubiquitous mitochondrial creatine kinase has 80% homology with the coding exons of sarcomeric mitochondrial creatine kinase. Two genes located near each other on chromosome 15 have been identified which encode identical mitochondrial creatine kinase proteins. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Human Creatine kinase MT 1A protein, His tag (active). GTX66950-pro

GTX66950-pro Image

3 μg of GTX66950-pro Human Creatine kinase MT 1A protein (active) by SDS-PAGE under reducing condition and visualized by coomassie blue stain

REFERENCE

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REVIEW

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SDS
Glycerol.pdf