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Human Creatine kinase (brain) protein, His tag

Human Creatine kinase (brain) protein, His tag. GTX00246-pro
Human Creatine kinase (brain) protein, His tag. GTX00246-pro
Human Creatine kinase (brain) protein, His tag. GTX00246-pro

Cat. No. GTX00246-pro

Application

 Functional Assay

Species

 Human
Package
10 μg ($299)
See all Creatine kinase (brain) protein products

APPLICATION

Application Note

Creatine Kinase, Brain (CKB) also known as CK-BB is a creatine kinase. CKB, consists of a homodimer of two identical brain-type CK-B subunits, is a cytoplasmic enzyme involved in cellular energy homeostasis, with certain fractions of the enzyme being bound to cell membranes, ATPases, and a variety of ATP-requiring enzymes in the cell. Besides, Creatine Kinase, Muscle (CKM) has been identified as an interactor of CKB, thus a binding ELISA assay was conducted to detect the interaction of recombinant human CKB and recombinant human CKM. Briefly, CKB were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100 μl were then transferred to CKM-coated microtiter wells and incubated for 2h at 37ºC. Wells were washed with PBST and incubated for 1h with anti-CKB pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37ºC. Finally, add 50 μl stop solution to the wells and read at 450nm immediately. The binding activity of CKB and CKM was in a dose dependent manner.

Observed MW

41 kDa.

PROPERTIES

Form

Lyophilized powder

Buffer

Reconstitute with 20mM Tris and 150mM NaCl to 0.1-1.0mg/ml. Do not vortex. Lyophilized from 20mM Tris, 150mM NaCl, 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose.

Preservative

ProClin 300

Storage

For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, store at -20ºC or below. After reconstitution, keep as concentrated solution. Avoid freeze-thaw cycles.

Region/Sequence

N-terminal His-Tag; Lys11~Leu367 (NP_001814.2)

Expression System

E. coli

Purity

> 95%

Endotoxin

< 1 EU/μg

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

creatine kinase B , B-CK , BCK , CKBB , CPK-B , HEL-211 , HEL-S-29

Background

The protein encoded by this gene is a cytoplasmic enzyme involved in energy homeostasis. The encoded protein reversibly catalyzes the transfer of phosphate between ATP and various phosphogens such as creatine phosphate. It acts as a homodimer in brain as well as in other tissues, and as a heterodimer with a similar muscle isozyme in heart. The encoded protein is a member of the ATP:guanido phosphotransferase protein family. A pseudogene of this gene has been characterized. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Human Creatine kinase (brain) protein, His tag. GTX00246-pro

GTX00246-pro Functional Assay Image

Functional ELISA analysis of GTX00246-pro Human Creatine kinase (brain) protein which can bind immobilized FUS protein.

Human Creatine kinase (brain) protein, His tag. GTX00246-pro

GTX00246-pro Image

WB analysis of GTX00246-pro Human Creatine kinase (brain) protein.

Human Creatine kinase (brain) protein, His tag. GTX00246-pro

GTX00246-pro Image

SDS-PAGE analysis of GTX00246-pro Human Creatine kinase (brain) protein.

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SDS
Proclin.pdf
Package List Price ($)
$ 299