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Human Cyclophilin F protein, His tag (active)

Human Cyclophilin F protein, His tag (active). GTX66958-pro

Cat No. GTX66958-pro

Application

Functional Assay

Species

Human
Package
100 μg ($259)

APPLICATION

Application Note

Specific activity is > 900 nmol/min/mg, and is defined as the amount of enzyme that cleaves 1nmole of suc-AAFP-PNA per minute at 37ºC in Tris-HCl (pH 8.0) using chymotrypsin.

PROPERTIES

Form

Liquid

Buffer

20 mM Tris (pH 7.5) with 1 mM DTT, 10% glycerol.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml

Region/Sequence

N-terminal His-Tag; Length: 199 a.a. Sequence: MGSSHHHHHH SSGLVPRGSH MCSKGSGDPS SSSSSGNPLV YLDVDANGKP LGRVVLELKA DVVPKTAENF RALCTGEKGF GYKGSTFHRV IPSFMCQAGD FTNHNGTGGK SIYGSRFPDE NFTLKHVGPG VLSMANAGPN TNGSQFFICT IKTDWLDGKH VVFGHVKEGM DVVKKIESFG SKSGRTSKKI VITDCGQLS

Expression System

E.coli

Purity

> 95% by SDS-PAGE.

Endotoxin

< 1.0 EU/μg (determined by LAL method)

Conjugation

Unconjugated

Note

For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

peptidylprolyl isomerase F , CYP3 , CyP-M , Cyp-D , CypD

Background

The protein encoded by this gene is a member of the peptidyl-prolyl cis-trans isomerase (PPIase) family. PPIases catalyze the cis-trans isomerization of proline imidic peptide bonds in oligopeptides and accelerate the folding of proteins. This protein is part of the mitochondrial permeability transition pore in the inner mitochondrial membrane. Activation of this pore is thought to be involved in the induction of apoptotic and necrotic cell death. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Human Cyclophilin F protein, His tag (active). GTX66958-pro

GTX66958-pro Image

3 μg of GTX66958-pro Human Cyclophilin F protein (active) by SDS-PAGE under reducing condition and visualized by coomassie blue stain

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Package List Price ($)
$ 259