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Human Cystatin D protein, His tag (active)

Human Cystatin D protein, His tag (active). GTX66965-pro

Cat. No. GTX66965-pro

Application

 Functional Assay

Species

 Human
Package
50 μg ($289)
See all Cystatin D protein products

APPLICATION

Application Note

The IC₅₀ value is < 2.0 nM. The inhibitory function of Cystatin 5 on protease activity of papain was measured by a fluorometric assay using Z-FR-AMC at pH 7.5 at 25ºC.

Observed MW

13.5-18 kDa.

PROPERTIES

Form

Liquid

Buffer

PBS, 10% Glycerol

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.5 mg/ml (Please refer to the vial label for the specific concentration.)

Region/Sequence

C-terminal His-Tag; Length: 123 a.a. Sequence: QSRTLAGGIH ATDLNDKSVQ RALDFAISEY NKVINKDEYY SRPLQVMAAY QQIVGGVNYY FNVKFGRTTC TKSQPNLDNC PFNDQPKLKE EEFCSFQINE VPWEDKISIL NYKCRKVHHH HHH

Expression System

Baculovirus

Purity

> 90% by SDS-PAGE.

Endotoxin

< 1.0 EU/μg (determined by LAL method)

Conjugation

Unconjugated

Note

For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

cystatin D

Background

The cystatin superfamily encompasses proteins that contain multiple cystatin-like sequences. Some of the members are active cysteine protease inhibitors, while others have lost or perhaps never acquired this inhibitory activity. There are three inhibitory families in the superfamily, including the type 1 cystatins (stefins), type 2 cystatins and the kininogens. The type 2 cystatin proteins are a class of cysteine proteinase inhibitors found in a variety of human fluids and secretions. The cystatin locus on chromosome 20 contains the majority of the type 2 cystatin genes and pseudogenes. This gene is located in the cystatin locus and encodes a protein found in saliva and tears. The encoded protein may play a protective role against proteinases present in the oral cavity. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Human Cystatin D protein, His tag (active). GTX66965-pro

GTX66965-pro Image

3 μg of GTX66965-pro Human Cystatin D protein (active) by SDS-PAGE under reducing condition and visualized by coomassie blue stain

REFERENCE

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SDS
PBS.pdf
Glycerol.pdf
Package List Price ($)
$ 289