GeneTex
  • Country / Location Selection

United States (US)

Human DGKE protein (active)

Human DGKE protein (active). GTX00146-pro
Human DGKE protein (active). GTX00146-pro
Human DGKE protein (active). GTX00146-pro

Cat No. GTX00146-pro

Application Functional Assay
Species Human
Package
10 μg ($319)
APPLICATION

Application Note

DGKe (Diacylglycerol kinase epsilon) is an enzyme that mainly involved in the regeneration of phosphatidylinositol (PI) from diacylglycerol in the PI-cycle during cell signal transduction. PDHA1 (Pyruvate dehydrogenase E1 component subunit alpha) has been proven to be an interactor of DGKe through Affinity Capture-MS. Thus a binding ELISA assay was conducted to detect the interaction of recombinant human DGKe and recombinant human PDHA. Briefly, DGKe were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100 μl DGKe were then transferred to PDHA-coated microtiter wells and incubated for 2h at 37ºC. Wells were washed with PBST and incubated for 1h with anti-DGKe pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37ºC. Finally, add 50 μl stop solution to the wells and read at 450nm immediately. The binding activity of DGKe and PDHA was in a dose dependent manner.

Observed MW

33 kDa.
PROPERTIES

Form

Lyophilized powder

Buffer

Lyophilized from 20 mM Tris (pH 8.0) with 150 mM NaCl, 1 mM EDTA, 1 mM DTT, 0.01% SKL, 5% Trehalose, Proclin300. Reconstitute with 20 mM Tris and 150 mM NaCl (pH 8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Storage

For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, store at -20ºC or below. After reconstitution, keep as concentrated solution.Avoid freeze-thaw cycles.

Region/Sequence

N-terminal His-Tag; Leu243~Gly502 (NP_003638.1)

Expression System

E. coli

Purity

> 95%

Endotoxin

< 1 EU/μg

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

diacylglycerol kinase epsilon,AHUS7,DAGK5,DAGK6,DGK,NPHS7

Background

Diacylglycerol kinases are thought to be involved mainly in the regeneration of phosphatidylinositol (PI) from diacylglycerol in the PI-cycle during cell signal transduction. When expressed in mammalian cells, DGK-epsilon shows specificity for arachidonyl-containing diacylglycerol. DGK-epsilon is expressed predominantly in testis. [provided by RefSeq, Jul 2008]

Database

DATA IMAGES
Human DGKE protein (active). GTX00146-pro

GTX00146-pro Functional Assay Image

Functional ELISA analysis of GTX00146-pro Human DGKE protein (active) which can bind immobilized PDHA protein.

Human DGKE protein (active). GTX00146-pro

GTX00146-pro Image

SDS-PAGE analysis of GTX00146-pro Human DGKE protein (active).

Human DGKE protein (active). GTX00146-pro

GTX00146-pro Image

WB analysis of GTX00146-pro Human DGKE protein (active).

REFERENCE
There are currently no references for Human DGKE protein (active) (GTX00146-pro). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for Human DGKE protein (active) (GTX00146-pro). Be the first to share your experience with this product.
Package List Price ($)
$ 319