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Human DHODH protein (active)

Human DHODH protein (active). GTX00200-pro
Human DHODH protein (active). GTX00200-pro
Human DHODH protein (active). GTX00200-pro

Cat No. GTX00200-pro

Application Functional Assay
Species Human
Package
10 μg ($289)
APPLICATION

Application Note

Dihydroorotate dehydrogenase (DHODH) is an enzyme which catalyzes the fourth enzymatic step, the ubiquinone-mediated oxidation of dihydroorotate to orotate, in de novo pyrimidine biosynthesis. This protein is a mitochondrial protein located on the outer surface of the inner mitochondrial membrane. As an enzyme associated with the electron transport chain, DHODH could link mitochondrial bioenergetics, cell proliferation, ROS production, and apoptosis in certain cell types. DHODH depletion also resulted in increased ROS production, decreased membrane potential and cell growth retardation. Besides, FK506 Binding Protein 8 (FKBP8) has been identified as an interactor of DHODH, thus a binding ELISA assay was conducted to detect the interaction of recombinant human DHODH and recombinant human FKBP8. Briefly, DHODH were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100 μl were then transferred to FKBP8-coated microtiter wells and incubated for 2h at 37ºC. Wells were washed with PBST and incubated for 1h with anti- DHODH pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37ºC. Finally, add 50 μl stop solution to the wells and read at 450nm immediately. The binding activity of DHODH and FKBP8 was in a dose dependent manner.

Observed MW

42 kDa.
PROPERTIES

Form

Lyophilized powder

Buffer

Lyophilized from 20 mM Tris (pH 8.0) with 150 mM NaCl, 1 mM EDTA, 1 mM DTT, 0.01% SKL, 5% Trehalose, Proclin300. Reconstitute with 20 mM Tris and 150 mM NaCl (pH 8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Storage

For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, store at -20ºC or below. After reconstitution, keep as concentrated solution.Avoid freeze-thaw cycles.

Region/Sequence

N-terminal His-Tag; Arg35~Asp392 (NP_001352.2)

Expression System

E. coli

Purity

> 90%

Endotoxin

< 1 EU/μg

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

dihydroorotate dehydrogenase (quinone),DHOdehase,POADS,URA1

Background

The protein encoded by this gene catalyzes the fourth enzymatic step, the ubiquinone-mediated oxidation of dihydroorotate to orotate, in de novo pyrimidine biosynthesis. This protein is a mitochondrial protein located on the outer surface of the inner mitochondrial membrane. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES
Human DHODH protein (active). GTX00200-pro

GTX00200-pro Functional Assay Image

Functional ELISA analysis of GTX00200-pro Human DHODH protein (active) which can bind immobilized FKBP8 protein.

Human DHODH protein (active). GTX00200-pro

GTX00200-pro Image

SDS-PAGE analysis of GTX00200-pro Human DHODH protein (active).

Human DHODH protein (active). GTX00200-pro

GTX00200-pro Image

WB analysis of GTX00200-pro Human DHODH protein (active).

REFERENCE
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REVIEW
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Package List Price ($)
$ 289