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Human DKK1 protein (active)

Human DKK1 protein (active). GTX00188-pro
Human DKK1 protein (active). GTX00188-pro
Human DKK1 protein (active). GTX00188-pro

Cat No. GTX00188-pro

Application Functional Assay
Species Human
Package
10 μg ($289)
APPLICATION

Application Note

Dickkopf-related protein 1 (DKK1) is a protein which is a member of the dickkopf family. It is a secreted protein with two cysteine rich regions and is involved in embryonic development through its inhibition of the Wnt signaling pathway. Dickkopf WNT signaling pathway inhibitor 1 (Dkk1) is a protein-coding gene that acts from the anterior visceral endoderm. The dickkopf protein encoded by DKK1 is an antagonistic inhibitor of the WNT signaling pathway that acts by isolating the CTNNb1 co-receptor so that it cannot aid in activating the WNT signaling pathway. DKK1 was also demonstrated to antagonize the Wnt/β-catenin pathway via a reduction in β-catenin and an increase in OCT4 expression. Besides, Catenin Beta1 (CTNNb1) has been identified as an interactor of DKK1 thus a binding ELISA assay was conducted to detect the interaction of recombinant human DKK1 and recombinant human CTNNb1. Briefly, DKK1 were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100 μl were then transferred to CTNNb1-coated microtiter wells and incubated for 2h at 37ºC. Wells were washed with PBST and incubated for 1h with anti-DKK1 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37ºC. Finally, add 50 μl stop solution to the wells and read at 450nm immediately. The binding activity of DKK1 and CTNNb1 was in a dose dependent manner.

Observed MW

33 kDa.
PROPERTIES

Form

Lyophilized powder

Buffer

Lyophilized from PBS (pH 7.4) with 0.01% SKL, 1 mM DTT, 5% Trehalose, Proclin300. Reconstitute with 10 mM PBS (pH 7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex

Storage

For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, store at -20ºC or below. After reconstitution, keep as concentrated solution.Avoid freeze-thaw cycles.

Region/Sequence

N-terminal His-Tag; Leu33~His266 (NP_036374.1)

Expression System

E. coli

Purity

> 95%

Endotoxin

< 1 EU/μg

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

dickkopf WNT signaling pathway inhibitor 1 , DKK-1 , SK

Background

This gene encodes a member of the dickkopf family of proteins. Members of this family are secreted proteins characterized by two cysteine-rich domains that mediate protein-protein interactions. The encoded protein binds to the LRP6 co-receptor and inhibits beta-catenin-dependent Wnt signaling. This gene plays a role in embryonic development and may be important in bone formation in adults. Elevated expression of this gene has been observed in numerous human cancers and this protein may promote proliferation, invasion and growth in cancer cell lines. [provided by RefSeq, Sep 2017]

Database

Research Area

DATA IMAGES
Human DKK1 protein (active). GTX00188-pro

GTX00188-pro Functional Assay Image

Functional ELISA analysis of GTX00188-pro Human DKK1 protein (active) which can bind immobilized CTNNb1 protein.

Human DKK1 protein (active). GTX00188-pro

GTX00188-pro Image

SDS-PAGE analysis of GTX00188-pro Human DKK1 protein (active).

Human DKK1 protein (active). GTX00188-pro

GTX00188-pro Image

WB analysis of GTX00188-pro Human DKK1 protein (active).

REFERENCE
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REVIEW
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Package List Price ($)
$ 289