20 mM Tris-HCl buffer (pH 8.0) with 0.15 M NaCl, 20% glycerol, 1 mM DTT.
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
0.5 mg/ml (Please refer to the vial label for the specific concentration.)
Full length protein, N-terminal His-Tag; MGSSHHHHHH SSGLVPRGSH MGSMLRGSAS STSMEKAKGK EWTSTEKSRE EDQQASNQPN SIALPGTSAK RTKEKMSIKG SKVLCPKKKA EHTDNPRPQK KIPIPPLPSK LPPVNLIHRD ILRAWCQQLK LSSKGQKLDA YKRLCAFAYP NQKDFPSTAK EAKIRKSLQK KLKVEKGETS LQSSETHPPE VALPPVGEPP ALENSTALLE GVNTVVVTTS APEALLASWA RISARARTPE AVESPQEASG VRWCVVHGKS LPADTDGWVH LQFHAGQAWV PEKQEGRVSA LFLLPASNFP PPHLEDNMLC PKCVHRNKVL IKSLQWE
> 85% by SDS-PAGE.
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
developmental pluripotency associated 4 , 2410091M23Rik
This gene encodes a nuclear factor that is involved in the maintenance of pluripotency in stem cells and essential for embryogenesis. The encoded protein has a scaffold-attachment factor A/B, acinus and PIAS (SAP) domain that binds DNA and is thought to modify chromatin. Mice with a homozygous knockout of the orthologous gene die during late embryonic development or within hours after birth. Knockout embryos are normal in size at embryonic day 18.5 but exhibit skeletal and lung tissue abnormalities. This gene, when mutated, is highly expressed in embryonal carcinomas, pluripotent germ cell tumors, and other cancers and is thought to play an important role in tumor progression. Multiple pseudogenes of this gene have been identified. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2017]