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Human DR5 protein (active)

Human DR5 protein (active). GTX00121-pro
Human DR5 protein (active). GTX00121-pro
Human DR5 protein (active). GTX00121-pro

Cat No. GTX00121-pro

Application Functional Assay
Species Human
Package
10 μg ($259)
APPLICATION

Application Note

TNFRSF10B (Tumor necrosis factor receptor superfamily member 10B) is a member of the TNF-receptor superfamily, and contains an intracellular death domain. By binding to certain ligand, this receptor transduces an apoptosis signal. A binding ELISA assay was conducted to detect the association of FAS with TNFa. Briefly, FAS were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100 μl FAS were then transferred to TNFa-coated microtiter wells and incubated for 2h at 37ºC. Wells were washed with PBST and incubated for 1h with anti-FAS pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37ºC. Finally, add 50 μl stop solution to the wells and read at 450nm immediately. The binding activity of FAS and TNFa was in a dose dependent manner.

Observed MW

53 kDa.
PROPERTIES

Form

Lyophilized powder

Buffer

Lyophilized from 20 mM Tris (pH 8.0) with 150 mM NaCl, 1 mM EDTA, 1 mM DTT, 0.01% SKL, 5% Trehalose, Proclin300. Reconstitute with 20 mM Tris and 150 mM NaCl (pH 8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Storage

For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, store at -20ºC or below. After reconstitution, keep as concentrated solution.Avoid freeze-thaw cycles.

Region/Sequence

N-terminal His and GST-Tag; Ser234~Ala435② (NP_003833.4)

Expression System

E. coli

Purity

> 97%

Endotoxin

< 1 EU/μg

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

TNF receptor superfamily member 10b,CD262,DR5,KILLER,KILLER/DR5,TRAIL-R2,TRAILR2,TRICK2,TRICK2A,TRICK2B,TRICKB,ZTNFR9

Background

The protein encoded by this gene is a member of the TNF-receptor superfamily, and contains an intracellular death domain. This receptor can be activated by tumor necrosis factor-related apoptosis inducing ligand (TNFSF10/TRAIL/APO-2L), and transduces an apoptosis signal. Studies with FADD-deficient mice suggested that FADD, a death domain containing adaptor protein, is required for the apoptosis mediated by this protein. Two transcript variants encoding different isoforms and one non-coding transcript have been found for this gene. [provided by RefSeq, Mar 2009]

Database

  • Gene ID: 8795 TNFRSF10B
  • UniProt: O14763 TNFRSF10B

Research Area

DATA IMAGES
Human DR5 protein (active). GTX00121-pro

GTX00121-pro Functional Assay Image

Functional ELISA analysis of GTX00121-pro Human DR5 protein (active) which can bind immobilized TNF alpha protein.

Human DR5 protein (active). GTX00121-pro

GTX00121-pro Image

WB analysis of GTX00121-pro Human DR5 protein (active).

Human DR5 protein (active). GTX00121-pro

GTX00121-pro Image

SDS-PAGE analysis of GTX00121-pro Human DR5 protein (active).

REFERENCE
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REVIEW
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Package List Price ($)
$ 259