GeneTex

  • Country / Location Selection

United States (US)

Human DUSP3 protein, His tag (active)

Human DUSP3 protein, His tag (active). GTX66975-pro

Cat. No. GTX66975-pro

Application

 Functional Assay

Species

 Human
Package
100 μg ($289)
See all DUSP3 protein products

APPLICATION

Application Note

Specific activity is > 1000 units/mg, and is defined as the amount of enzyme that hydrolyze 1.0 nmoles of p-nitrophenyl phosphate (pNPP) per minute at pH 7.5 at 37ºC.

PROPERTIES

Form

Liquid

Buffer

20mM Tris-HCl buffer (pH8.0), 10% Glycerol, 2mM DTT, 0.15M NaCl

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Region/Sequence

Full length protein, N-terminal His-Tag; Length: 205 a.a. Sequence: MGSSHHHHHH SSGLVPRGSH MSGSFELSVQ DLNDLLSDGS GCYSLPSQPC NEVTPRIYVG NASVAQDIPK LQKLGITHVL NAAEGRSFMH VNTNANFYKD SGITYLGIKA NDTQEFNLSA YFERAADFID QALAQKNGRV LVHCREGYSR SPTLVIAYLM MRQKMDVKSA LSIVRQNREI GPNDGFLAQL CQLNDRLAKE GKLKP

Expression System

E. coli

Purity

> 95% by SDS-PAGE.

Conjugation

Unconjugated

Note

For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

dual specificity phosphatase 3 , VHR

Background

The protein encoded by this gene is a member of the dual specificity protein phosphatase subfamily. These phosphatases inactivate their target kinases by dephosphorylating both the phosphoserine/threonine and phosphotyrosine residues. They negatively regulate members of the mitogen-activated protein (MAP) kinase superfamily (MAPK/ERK, SAPK/JNK, p38), which are associated with cellular proliferation and differentiation. Different members of the family of dual specificity phosphatases show distinct substrate specificities for various MAP kinases, different tissue distribution and subcellular localization, and different modes of inducibility of their expression by extracellular stimuli. This gene maps in a region that contains the BRCA1 locus which confers susceptibility to breast and ovarian cancer. Although DUSP3 is expressed in both breast and ovarian tissues, mutation screening in breast cancer pedigrees and in sporadic tumors was negative, leading to the conclusion that this gene is not BRCA1. [provided by RefSeq, Jul 2008]

Database

DATA IMAGES

Human DUSP3 protein, His tag (active). GTX66975-pro

GTX66975-pro Image

3 μg of GTX66975-pro Human DUSP3 protein (active) by SDS-PAGE under reducing condition and visualized by coomassie blue stain

REFERENCE

There are currently no references for Human DUSP3 protein, His tag (active) (GTX66975-pro). Be the first to share your publications with this product.
Submit a Reference

REVIEW

There are currently no reviews for Human DUSP3 protein, His tag (active) (GTX66975-pro). Be the first to share your experience with this product.
Submit a Review
SDS
Glycerol.pdf
Package List Price ($)
$ 289