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Human ECHS1 protein, His tag (active)

Human ECHS1 protein, His tag (active). GTX66976-pro

Cat. No. GTX66976-pro

Application

 Functional Assay

Species

 Human
Package
20 μg ($219)
See all ECHS1 protein products

APPLICATION

Application Note

Specific activity is > 150 units/mg, and is defined as the amount of enzyme that hydrolyze 1.0 μmole of crotonoyl-CoA to hydroxybutyryl-CoA per minute per minute at pH 7.5 at 25ºC.

PROPERTIES

Form

Liquid

Buffer

20mM Tris-HCl buffer (pH8.0), 1mM DTT, 20% Glycerol, 100mM NaCl

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Region/Sequence

N-terminal His-Tag; Length: 284 a.a. Sequence: MGSSHHHHHH SSGLVPRGSH MASGANFEYI IAEKRGKNNT VGLIQLNRPK ALNALCDGLI DELNQALKIF EEDPAVGAIV LTGGDKAFAA GADIKEMQNL SFQDCYSSKF LKHWDHLTQV KKPVIAAVNG YAFGGGCELA MMCDIIYAGE KAQFAQPEIL IGTIPGAGGT QRLTRAVGKS LAMEMVLTGD RISAQDAKQA GLVSKICPVE TLVEEAIQCA EKIASNSKIV VAMAKESVNA AFEMTLTEGS KLEKKLFYST FATDDRKEGM TAFVEKRKAN FKDQ

Expression System

E. coli

Purity

> 95% by SDS-PAGE.

Conjugation

Unconjugated

Note

For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

enoyl-CoA hydratase, short chain 1 , ECHS1D , SCEH

Background

The protein encoded by this gene functions in the second step of the mitochondrial fatty acid beta-oxidation pathway. It catalyzes the hydration of 2-trans-enoyl-coenzyme A (CoA) intermediates to L-3-hydroxyacyl-CoAs. The gene product is a member of the hydratase/isomerase superfamily. It localizes to the mitochondrial matrix. Transcript variants utilizing alternative transcription initiation sites have been described in the literature. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Human ECHS1 protein, His tag (active). GTX66976-pro

GTX66976-pro Image

3 μg of GTX66976-pro Human ECHS1 protein (active) by SDS-PAGE under reducing condition and visualized by coomassie blue stain

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Package List Price ($)
$ 219