APPLICATION
Application Note
Endothelial gland-derived VEGF (EG-VEGF) is an angiogenic protein that is structurally unrelated to VEGF. It is expressed in steroidogenic tissues such as adrenal gland, ovary, testis, and placenta. Like VEGF it can induce fenestrae in endothelial cells. To test the effect of EG-VEGF on cell proliferation of ECV-304 endothelium cell line, cells were seeded into triplicate wells of 96-well plates at a density of 5000 cells/well and allowed to attach overnight, then the medium was replaced with serum-free standard DMEM prior to the addition of various concentrations of EG-VEGF. After incubated for 72h, cells were observed by inverted microscope and cell proliferation was measured by Cell Counting Kit-8 (CCK-8). Briefly, 10 μl of CCK-8 solution was added to each well of the plate, then measure the absorbance at 450nm using a microplate reader after incubating the plate for 1-4 hours at 37ºC.
Observed MW
44 kDa.
PROPERTIES
Form
Lyophilized powder
Buffer
Reconstitute with 20mM Tris and 150mM NaCl to 0.1-1.0mg/ml. Do not vortex. Lyophilized from 20mM Tris, 150mM NaCl, 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose.
Preservative
ProClin 300
Storage
For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, store at -20ºC or below. After reconstitution, keep as concentrated solution. Avoid freeze-thaw cycles.
Region/Sequence
Full length protein, N-terminal GST-Tag; Met1~Phe105 (NP_115790.1)
Expression System
E. coli
Purity
> 90%
Endotoxin
< 1 EU/μg
Conjugation
Unconjugated
Note
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
Synonyms
prokineticin 1 , EGVEGF , PK1 , PRK1
Background
The protein encoded by this gene induces proliferation, migration, and fenestration (the formation of membrane discontinuities) in capillary endothelial cells derived from endocrine glands. It has little or no effect on a variety of other endothelial and non-endothelial cell types. Its expression is restricted to the steroidogenic glands (ovary, testis, adrenal, and placenta), is induced by hypoxia, and often complementary to the expression of vascular endothelial growth factor (VEGF), suggesting that these molecules function in a coordinated manner. [provided by RefSeq, Sep 2011]
Database
Research Area
DATA IMAGES
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GTX00186-pro Functional Assay Image
Cell proliferation effect of GTX00186-pro Human EG-VEGF protein (active). Cell viability was measured by Cell Counting Kit-8 (CCK-8).
(A) Unstimulated ECV-304 cells cultured in RPMI-1640 for 96hrs.
(B) ECV-304 cells cultured in RPMI-1640, stimulated with 10 ng/ml VEGF121 for 96hrs.
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GTX00186-pro Image
WB analysis of GTX00186-pro Human EG-VEGF protein (active).
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GTX00186-pro Image
SDS-PAGE analysis of GTX00186-pro Human EG-VEGF protein (active).
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REFERENCE
There are currently no references for Human EG-VEGF protein, GST tag (active) (GTX00186-pro). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for Human EG-VEGF protein, GST tag (active) (GTX00186-pro). Be the first to share your experience with this product.
