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Human EG-VEGF protein (active)

Human EG-VEGF protein (active). GTX00186-pro
Human EG-VEGF protein (active). GTX00186-pro
Human EG-VEGF protein (active). GTX00186-pro

Cat No. GTX00186-pro

Application Functional Assay
Species Human
Package
10 μg ($309)
APPLICATION

Application Note

Endothelial gland-derived VEGF (EG-VEGF) is an angiogenic protein that is structurally unrelated to VEGF. It is expressed in steroidogenic tissues such as adrenal gland, ovary, testis, and placenta. Like VEGF it can induce fenestrae in endothelial cells. To test the effect of EG-VEGF on cell proliferation of ECV-304 endothelium cell line, cells were seeded into triplicate wells of 96-well plates at a density of 5000 cells/well and allowed to attach overnight, then the medium was replaced with serum-free standard DMEM   prior to the addition of various concentrations of EG-VEGF. After incubated for 72h,  cells were observed by inverted microscope and cell proliferation was measured by Cell Counting Kit-8 (CCK-8). Briefly, 10 μl of CCK-8 solution was added to each well of the plate, then measure the absorbance at 450nm using a microplate reader after incubating the plate for 1-4 hours at 37ºC.

Observed MW

44 kDa.
PROPERTIES

Form

Lyophilized powder

Buffer

Lyophilized from 20 mM Tris (pH 8.0) with 150 mM NaCl, 1 mM EDTA, 1 mM DTT, 0.01% SKL, 5% Trehalose, Proclin300. Reconstitute with 20 mM Tris and 150 mM NaCl (pH 8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Storage

For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, store at -20ºC or below. After reconstitution, keep as concentrated solution.Avoid freeze-thaw cycles.

Region/Sequence

Full length protein, N-terminal GST-Tag; Met1~Phe105 (NP_115790.1)

Expression System

E. coli

Purity

> 90%

Endotoxin

< 1 EU/μg

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

prokineticin 1,EGVEGF,PK1,PRK1

Background

The protein encoded by this gene induces proliferation, migration, and fenestration (the formation of membrane discontinuities) in capillary endothelial cells derived from endocrine glands. It has little or no effect on a variety of other endothelial and non-endothelial cell types. Its expression is restricted to the steroidogenic glands (ovary, testis, adrenal, and placenta), is induced by hypoxia, and often complementary to the expression of vascular endothelial growth factor (VEGF), suggesting that these molecules function in a coordinated manner. [provided by RefSeq, Sep 2011]

Database

Research Area

DATA IMAGES
Human EG-VEGF protein (active). GTX00186-pro

GTX00186-pro Functional Assay Image

Cell proliferation effect of GTX00186-pro Human EG-VEGF protein (active). Cell viability was measured by Cell Counting Kit-8 (CCK-8).
(A) Unstimulated ECV-304 cells cultured in RPMI-1640 for 96hrs.
(B) ECV-304 cells cultured in RPMI-1640, stimulated with 10 ng/ml VEGF121 for 96hrs.

Human EG-VEGF protein (active). GTX00186-pro

GTX00186-pro Image

WB analysis of GTX00186-pro Human EG-VEGF protein (active).

Human EG-VEGF protein (active). GTX00186-pro

GTX00186-pro Image

SDS-PAGE analysis of GTX00186-pro Human EG-VEGF protein (active).

REFERENCE
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REVIEW
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