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Human EGF protein, His tag (active)

Human EGF protein, His tag (active). GTX00114-pro
Human EGF protein, His tag (active). GTX00114-pro
Human EGF protein, His tag (active). GTX00114-pro
Human EGF protein, His tag (active). GTX00114-pro

Cat. No. GTX00114-pro

Application

 Functional Assay

Species

 Human
Package
10 μg ($199)
See all EGF protein products

APPLICATION

Application Note

Epidermal growth factor (EGF) is a growth factor that stimulates cell growth,  proliferation, and differentiation by binding to its receptor EGFR. To test the effect of EGF on cell proliferation of 3T3 fibroblasts, 3T3 cells were seeded into triplicate wells of 96-well plates at a density of 2000 cells/well and allowed to attach overnight, then the medium was replaced with serum-free standard DMEM  prior to the addition of various concentrations of EGF. After incubated for 72h,   cells were observed by inverted microscope and cell proliferation was measured by Cell Counting Kit-8 (CCK-8). Briefly, 10 μl of CCK-8 solution was added to each well of the plate, then measure the absorbance at 450nm using a microplate reader after incubating the plate for 1-4 hours at 37ºC . Cell proliferation of 3T3 cells after incubation with EGF for 72h observed by inverted microscope.

Observed MW

10 kDa.

PROPERTIES

Form

Lyophilized powder

Buffer

Reconstitute with 20mM Tris and 150mM NaCl to 0.1-1.0mg/ml. Do not vortex. Lyophilized from 20mM Tris, 150mM NaCl, 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose.

Preservative

ProClin 300

Storage

For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, store at -20ºC or below. After reconstitution, keep as concentrated solution. Avoid freeze-thaw cycles.

Region/Sequence

N-terminal His-Tag; Asn971~Arg1023 (NP_001171601.1)

Expression System

E. coli

Purity

> 90%

Endotoxin

< 1 EU/μg

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

epidermal growth factor , HOMG4 , URG

Background

This gene encodes a member of the epidermal growth factor superfamily. The encoded preproprotein is proteolytically processed to generate the 53-amino acid epidermal growth factor peptide. This protein acts a potent mitogenic factor that plays an important role in the growth, proliferation and differentiation of numerous cell types. This protein acts by binding with high affinity to the cell surface receptor, epidermal growth factor receptor. Defects in this gene are the cause of hypomagnesemia type 4. Dysregulation of this gene has been associated with the growth and progression of certain cancers. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed. [provided by RefSeq, Jan 2016]

Database

Research Area

DATA IMAGES

Human EGF protein, His tag (active). GTX00114-pro

GTX00114-pro Functional Assay Image

The dose effect curve of GTX00114-pro Human EGF protein (active) on 3T3 cells. After incubation of Human EGF protein (active) for 72 hrs, cell proliferation was measured by Cell Counting Kit-8 (CCK-8).

Human EGF protein, His tag (active). GTX00114-pro

GTX00114-pro Functional Assay Image

Cell proliferation effect of GTX00114-pro Human EGF protein (active).
(A) Unstimulated 3T3 cells cultured in serum-free DMEM for 72hrs.
(B) 3T3 cells cultured in DMEM, stimulated with 1 ng/ml EGF for 72hrs.

Human EGF protein, His tag (active). GTX00114-pro

GTX00114-pro Image

SDS-PAGE analysis of GTX00114-pro Human EGF protein (active).

Human EGF protein, His tag (active). GTX00114-pro

GTX00114-pro Image

WB analysis of GTX00114-pro Human EGF protein (active).

REFERENCE

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REVIEW

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SDS
Proclin.pdf
Package List Price ($)
$ 199