Human EGF protein (active)

Cat. No. GTX136910-pro

Application	Functional Assay
Species	Human
Package	500 μg ($199)

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Datasheet

View product citations for antibody GTX136910-pro on CiteAb

PRODUCT

Summary

Epidermal Growth Factor (EGF) is a 53-a.a. peptide cleaved from a 1207 a.a. transmembrane glycoprotein precursor. Its binding to the EGF receptor (EGFR) affects cell proliferation and differentiation via downstream effectors.

APPLICATION

Application Note

The determined ED50 is ≤ 0.1 ng/mL, which corresponds to a specific activity of ≥ 1 x 107 units/mg and is comparable to similar commercial products. The biological activity of this recombinant human EGF protein was analyzed using the BALB/c 3T3 Clone A31 mouse embryonic fibroblast cell proliferation assay (Rubin, J.S. et al. (1991)).

Observed MW

12 kDa.

PROPERTIES

Form

Lyophilized powder

Buffer

Reconstitute with distilled water to 0.1 mg/ml. Lyophilized from 50mM PBS, 100mM NaCl, 5% Trehalose.

Preservative

No Preservative

Storage

Store at 4ºC or below. After reconstitution, keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. For long-term storage after reconstitution, aliquot and store at -70ºC or below. Do not vortex.

Region/Sequence

Mature human EGF protein ( Asn971~Arg1023 of NP_001954.2)

Expression System

E. Coli

Purity

> 98%

Endotoxin

< 0.1 EU/μg

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

epidermal growth factor , HOMG4 , URG

Cellular Localization

Membrane; Single-pass type I membrane protein

Background

This gene encodes a member of the epidermal growth factor superfamily. The encoded preproprotein is proteolytically processed to generate the 53-amino acid epidermal growth factor peptide. This protein acts a potent mitogenic factor that plays an important role in the growth, proliferation and differentiation of numerous cell types. This protein acts by binding with high affinity to the cell surface receptor, epidermal growth factor receptor. Defects in this gene are the cause of hypomagnesemia type 4. Dysregulation of this gene has been associated with the growth and progression of certain cancers. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed. [provided by RefSeq, Jan 2016]

Database

Gene ID: 1950 EGF
UniProt: P01133 EGF

Research Area

DATA IMAGES

GTX136910-pro Functional Assay Image

Serial dilutions of Human EGF protein (active) were added to BALB/c 3T3 Clone A31 cells. Cell proliferation was measured and the linear portion of the curve was used to calculate the ED50.

GTX136910-pro Image

1μg of GTX136910-pro Human EGF protein (active) was analyzed using SDS-PAGE and stained with coomassie blue and captured by monochrome camera.

REFERENCE

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REVIEW

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Package

List Price ($)

500 μg

$ 199

Qty