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Human ENO3 protein, His tag (active)

Human ENO3 protein, His tag (active). GTX66981-pro

Cat No. GTX66981-pro

Application

Functional Assay

Species

Human
Package
50 μg ($359)

APPLICATION

Application Note

Specific activity is > 5000 pmol/min/μg, and was obtained by measuring the decrease of NAD in absorbance at 340 nm resulting from NADH at pH 6.5 at 37ºC.

PROPERTIES

Form

Liquid

Buffer

20 mM Tris-HCl buffer (pH 8.0) with 1 mM DTT, 20% glycerol, 100 mM NaCl.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.5 mg/ml

Region/Sequence

Full length protein, N-terminal His-Tag; Length: 454 a.a. Sequence: MGSSHHHHHH SSGLVPRGSH MAMQKIFARE ILDSRGNPTV EVDLHTAKGR FRAAVPSGAS TGIYEALELR DGDKGRYLGK GVLKAVENIN STLGPALLQK KLSVADQEKV DKFMIELDGT ENKSKFGANA ILGVSLAVCK AGAAEKGVPL YRHIADLAGN PDLILPVPAF NVINGGSHAG NKLAMQEFMI LPVGASSFKE AMRIGAEVYH HLKGVIKAKY GKDATNVGDE GGFAPNILEN NEALELLKTA IQAAGYPDKV VIGMDVAASE FYRNGKYDLD FKSPDDPARH ITGEKLGELY KSFIKNYPVV SIEDPFDQDD WATWTSFLSG VNIQIVGDDL TVTNPKRIAQ AVEKKACNCL LLKVNQIGSV TESIQACKLA QSNGWGVMVS HRSGETEDTF IADLVVGLCT GQIKTGAPCR SERLAKYNQL MRIEEALGDK AIFAGRKFRN PKAK

Expression System

E.coli

Purity

> 95% by SDS-PAGE.

Conjugation

Unconjugated

Note

For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

enolase 3 , GSD13 , MSE

Background

This gene encodes one of the three enolase isoenzymes found in mammals. This isoenzyme is found in skeletal muscle cells in the adult where it may play a role in muscle development and regeneration. A switch from alpha enolase to beta enolase occurs in muscle tissue during development in rodents. Mutations in this gene have be associated glycogen storage disease. Alternatively spliced transcript variants encoding different isoforms have been described.[provided by RefSeq, Jul 2010]

Database

Research Area

DATA IMAGES

Human ENO3 protein, His tag (active). GTX66981-pro

GTX66981-pro Image

3 μg of GTX66981-pro Human ENO3 protein (active) by SDS-PAGE under reducing condition and visualized by coomassie blue stain

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Package List Price ($)
$ 359