Cell proliferation effect of GTX00422-pro Human EPO protein (active), cell viability was measured by cell Counting Kit-8 (CCK-8).
(A) Unstimulated TF-1 cells cultured in RPMI-1640 for 72hrs.
(B) TF-1 cells cultured in RPMI-1640, stimulated with 5 ng/ml EPO for 72hrs.

Indirect ELISA analysis was performed by coating the plate with recombinant E.coli expressed, full-length human EPO protein, His and GST tag (active) (GTX00422-pro) (500-7.81 ng/mL). Coated protein was probed with EPO antibody (GTX100813) (1 μg/mL). Goat anti-rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) was used to detect the bound primary antibody.

WB analysis of GTX00422-pro Human EPO protein (active).

SDS-PAGE analysis of GTX00422-pro Human EPO protein (active).