GeneTex
  • Country / Location Selection

United States (US)

Human EPO protein, His tag (active)

Human EPO protein, His tag (active). GTX66983-pro

Cat No. GTX66983-pro

Application

Functional Assay

Species

Human
Package
50 μg ($289)

APPLICATION

Application Note

Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. The ED₅₀ for this effect is equal to or less than 0.5 ng/ml.

Observed MW

28-40 kDa.

PROPERTIES

Form

Liquid

Buffer

PBS (pH 7.4) with 10% glycerol.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.5 mg/ml

Region/Sequence

C-terminal His-Tag; Length: 174 a.a. Sequence: APPRLICDSR VLERYLLEAK EAENITTGCA EHCSLNENIT VPDTKVNFYA WKRMEVGQQA VEVWQGLALL SEAVLRGQAL LVNSSQPWEP LQLHVDKAVS GLRSLTTLLR ALRAQKEAIS PPDAASAAPL RTITADTFRK LFRVYSNFLR GKLKLYTGEA CRTGDRLEHH HHHH

Expression System

Baculovirus

Purity

> 95% by SDS-PAGE.

Endotoxin

< 1.0 EU/μg (determined by LAL method)

Conjugation

Unconjugated

Note

For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

erythropoietin , DBAL , ECYT5 , EP , MVCD2

Background

This gene encodes a secreted, glycosylated cytokine composed of four alpha helical bundles. The encoded protein is mainly synthesized in the kidney, secreted into the blood plasma, and binds to the erythropoietin receptor to promote red blood cell production, or erythropoiesis, in the bone marrow. Expression of this gene is upregulated under hypoxic conditions, in turn leading to increased erythropoiesis and enhanced oxygen-carrying capacity of the blood. Expression of this gene has also been observed in brain and in the eye, and elevated expression levels have been observed in diabetic retinopathy and ocular hypertension. Recombinant forms of the encoded protein exhibit neuroprotective activity against a variety of potential brain injuries, as well as antiapoptotic functions in several tissue types, and have been used in the treatment of anemia and to enhance the efficacy of cancer therapies. [provided by RefSeq, Aug 2017]

Database

Research Area

DATA IMAGES

Human EPO protein, His tag (active). GTX66983-pro

GTX66983-pro Image

3 μg of GTX66983-pro Human EPO protein (active) by SDS-PAGE under reducing condition and visualized by coomassie blue stain

REFERENCE

There are currently no references for Human EPO protein, His tag (active) (GTX66983-pro). Be the first to share your publications with this product.

REVIEW

There are currently no reviews for Human EPO protein, His tag (active) (GTX66983-pro). Be the first to share your experience with this product.
SDS
PBS.pdf
Glycerol.pdf