APPLICATION
Application Note
Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. The ED₅₀ for this effect is equal to or less than 0.5 ng/ml.
Observed MW
28-40 kDa.
PROPERTIES
Form
Liquid
Buffer
PBS, 10% Glycerol
Preservative
No preservative
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.5 mg/ml (Please refer to the vial label for the specific concentration.)
Region/Sequence
C-terminal His-Tag; Length: 174 a.a. Sequence: APPRLICDSR VLERYLLEAK EAENITTGCA EHCSLNENIT VPDTKVNFYA WKRMEVGQQA VEVWQGLALL SEAVLRGQAL LVNSSQPWEP
LQLHVDKAVS GLRSLTTLLR ALRAQKEAIS PPDAASAAPL RTITADTFRK LFRVYSNFLR GKLKLYTGEA CRTGDRLEHH HHHH
Expression System
Baculovirus
Purity
> 95% by SDS-PAGE.
Endotoxin
< 1.0 EU/μg (determined by LAL method)
Conjugation
Unconjugated
Note
For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
Synonyms
erythropoietin , DBAL , ECYT5 , EP , MVCD2
Background
This gene encodes a secreted, glycosylated cytokine composed of four alpha helical bundles. The encoded protein is mainly synthesized in the kidney, secreted into the blood plasma, and binds to the erythropoietin receptor to promote red blood cell production, or erythropoiesis, in the bone marrow. Expression of this gene is upregulated under hypoxic conditions, in turn leading to increased erythropoiesis and enhanced oxygen-carrying capacity of the blood. Expression of this gene has also been observed in brain and in the eye, and elevated expression levels have been observed in diabetic retinopathy and ocular hypertension. Recombinant forms of the encoded protein exhibit neuroprotective activity against a variety of potential brain injuries, as well as antiapoptotic functions in several tissue types, and have been used in the treatment of anemia and to enhance the efficacy of cancer therapies. [provided by RefSeq, Aug 2017]
Database
Research Area
DATA IMAGES
|
GTX66983-pro Image
3 μg of GTX66983-pro Human EPO protein (active) by SDS-PAGE under reducing condition and visualized by coomassie blue stain
|
REFERENCE
There are currently no references for Human EPO protein, His tag (active) (GTX66983-pro). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for Human EPO protein, His tag (active) (GTX66983-pro). Be the first to share your experience with this product.