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Human FGF21 protein, His tag (active)

Human FGF21 protein, His tag (active). GTX01258-pro
Human FGF21 protein, His tag (active). GTX01258-pro

Cat No. GTX01258-pro

Application

Functional Assay

Species

Human
Package
50 μg ($279),
20 μg ($199)

APPLICATION

Application Note

Measured in a cell proliferation assay using NIH-3T3 cells. The ED₅₀ for this effect is typically 0.1-1 μg/ml in the presence of 2.5 μg/ml of recombinant mouse Klotho β protein.

Observed MW

23 kDa.

PROPERTIES

Form

Lyophilized powder

Buffer

Batch dependent (please contact us for details)

Storage

Store at -20ºC or below. After reconstitution, keep as concentrated solution. Avoid freeze-thaw cycles.

Region/Sequence

N-terminal poly His tagged; His29-Ser209 of Human FGF21 protein (NP_061986.1)

Expression System

E. coli

Purity

> 95% by SDS-PAGE.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

FGF21

Background

Theis gene encodes a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities and are involved in a variety of biological processes. This protein is a secreted endocrine factor that functions as a major metabolic regulator. The encoded protein stimulates the uptake of glucose in adipose tissue. [provided by RefSeq, Mar 2016]

Database

Research Area

DATA IMAGES

Human FGF21 protein, His tag (active). GTX01258-pro

GTX01258-pro Functional Assay Image

The protein activity of GTX01258-pro Human FGF21 protein was measured in a cell proliferation assay using NIH-3T3 cells.
ED₅₀ : 0.1-1 μg/ml in the presence of 2.5 μg/ml of recombinant mouse Klotho β protein

Human FGF21 protein, His tag (active). GTX01258-pro

GTX01258-pro Image

SDS-PAGE of 5 μg GTX01258-pro Human FGF21 protein, His tag (active).

REFERENCE

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REVIEW

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