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Human FGF23 protein (active)

Human FGF23 protein (active). GTX00257-pro
Human FGF23 protein (active). GTX00257-pro
Human FGF23 protein (active). GTX00257-pro

Cat No. GTX00257-pro

Application Functional Assay
Species Human
Package
10 μg ($289)
APPLICATION

Application Note

Fibroblast growth factor 23 or FGF23 is a member of the fibroblast growth factor (FGF) family which is responsible for phosphate and vitamin D metabolism. The main function of FGF23 seems to be regulation of phosphate concentration in plasma. FGF23 decreases the reabsorption and increases excretion of phosphate and suppress 1-alpha-hydroxylase, reducing its ability to activate vitamin D and subsequently impairing calcium absorption. Besides, Fibroblast Growth Factor Receptor 2 (FGFR2) has been identified as an interactor of FGF23, thus a binding ELISA assay was conducted to detect the interaction of recombinant human FGF23 and recombinant human FGFR2. Briefly, FGF23 were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100 μl were then transferred to FGFR2-coated microtiter wells and incubated for 2h at 37ºC. Wells were washed with PBST and incubated for 1h with anti-FGF23 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37ºC. Finally, add 50 μl stop solution to the wells and read at 450nm immediately. The binding activity of FGF23 and FGFR2 was in a dose dependent manner.

Observed MW

11 kDa.
PROPERTIES

Form

Lyophilized powder

Buffer

Lyophilized from 20 mM Tris (pH 8.0) with 150 mM NaCl, 1 mM EDTA, 1 mM DTT, 0.01% SKL, 5% Trehalose, Proclin300. Reconstitute with 20 mM Tris and 150 mM NaCl (pH 8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Storage

For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, store at -20ºC or below. After reconstitution, keep as concentrated solution.Avoid freeze-thaw cycles.

Region/Sequence

N-terminal His-Tag; Asp79~Arg160 (NP_065689.1)

Expression System

E. coli

Purity

> 95%

Endotoxin

< 1 EU/μg

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

fibroblast growth factor 23,ADHR,FGFN,HFTC2,HPDR2,HYPF,PHPTC

Background

This gene encodes a member of the fibroblast growth factor family of proteins, which possess broad mitogenic and cell survival activities and are involved in a variety of biological processes. The product of this gene regulates phosphate homeostasis and transport in the kidney. The full-length, functional protein may be deactivated via cleavage into N-terminal and C-terminal chains. Mutation of this cleavage site causes autosomal dominant hypophosphatemic rickets (ADHR). Mutations in this gene are also associated with hyperphosphatemic familial tumoral calcinosis (HFTC). [provided by RefSeq, Feb 2013]

Database

Research Area

DATA IMAGES
Human FGF23 protein (active). GTX00257-pro

GTX00257-pro Functional Assay Image

Functional ELISA analysis of GTX00257-pro Human FGF23 protein (active) which can bind immobilized FGFR2 protein.

Human FGF23 protein (active). GTX00257-pro

GTX00257-pro Image

WB analysis of GTX00257-pro Human FGF23 protein (active).

Human FGF23 protein (active). GTX00257-pro

GTX00257-pro Image

SDS-PAGE analysis of GTX00257-pro Human FGF23 protein (active).

REFERENCE
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REVIEW
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Package List Price ($)
$ 289