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Human FGF6 protein (active)

Human FGF6 protein (active). GTX00224-pro
Human FGF6 protein (active). GTX00224-pro
Human FGF6 protein (active). GTX00224-pro

Cat No. GTX00224-pro

Application

Functional Assay

Species

Human
Package
10 μg ($269)

APPLICATION

Application Note

Fibroblast growth factor 6 (FGF6) is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. It may also play a importent role in muscle regeneration or differentiation. Besides, Fibroblast Growth Factor Receptor 1 (FGFR1) has been identified as an interactor of FGF6, thus a binding ELISA assay was conducted to detect the interaction of recombinant human FGF6 and recombinant human FGFR1. Briefly, FGF6 were diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100 μl were then transferred to FGFR1-coated microtiter wells and incubated for 2h at 37ºC. Wells were washed with PBST and incubated for 1h with anti-FGF6 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37ºC. Finally, add 50 μl stop solution to the wells and read at 450nm immediately. The binding activity of FGF6 and FGFR1 was in a dose dependent manner.

Observed MW

38 kDa.

PROPERTIES

Form

Lyophilized powder

Buffer

Lyophilized from 20 mM Tris (pH 8.0) with 150 mM NaCl, 1 mM EDTA, 1 mM DTT, 0.01% SKL, 5% Trehalose, Proclin300. Reconstitute with 20 mM Tris and 150 mM NaCl (pH 8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Storage

For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, store at -20ºC or below. After reconstitution, keep as concentrated solution.Avoid freeze-thaw cycles.

Region/Sequence

N-terminal His and GST-Tag; Gly41~Ile208 (NP_066276.2)

Expression System

E. coli

Purity

> 90%

Endotoxin

< 1 EU/μg

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

fibroblast growth factor 6 , HBGF-6 , HST2

Background

The protein encoded by this gene is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. This gene displayed oncogenic transforming activity when transfected into mammalian cells. The mouse homolog of this gene exhibits a restricted expression profile predominantly in the myogenic lineage, which suggested a role in muscle regeneration or differentiation. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Human FGF6 protein (active). GTX00224-pro

GTX00224-pro Functional Assay Image

Functional ELISA analysis of GTX00224-pro Human FGF6 protein (active) which can bind immobilized FGFR1 protein.

Human FGF6 protein (active). GTX00224-pro

GTX00224-pro Image

SDS-PAGE analysis of GTX00224-pro Human FGF6 protein (active).

Human FGF6 protein (active). GTX00224-pro

GTX00224-pro Image

WB analysis of GTX00224-pro Human FGF6 protein (active).

REFERENCE

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REVIEW

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Package List Price ($)
$ 269