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Human FNDC5 protein, His tag (active)

Human FNDC5 protein, His tag (active). GTX00150-pro
Human FNDC5 protein, His tag (active). GTX00150-pro
Human FNDC5 protein, His tag (active). GTX00150-pro
Human FNDC5 protein, His tag (active). GTX00150-pro

Cat. No. GTX00150-pro

Application

 Functional Assay

Species

 Human
Package
10 μg ($309)
See all FNDC5 protein products

APPLICATION

Application Note

Fibronectin type III domain-containing protein 5, the precursor of irisin, is a protein that is encoded by the FNDC5  gene. It was reported that FNDC5 significantly decreased cell number, migration and viability through apoptosis in malignant MDA-MB-231 cells. Thus MDA-MB-231 cells were seeded overnight at a density of 5000 cells/well, and treated with or without various concentrations of FNDC5 for 48h, then MDA-MB-231 cells were observed by inverted microscope and cell viability was measured by Cell Counting Kit-8 (CCK-8). Briefly, 10 μl of CCK-8 solution was added to each well of the plate, then measure the absorbance at 450nm using a microplate reader after incubating the plate for 1-4 hours at 37ºC. Cell apoptosis of MDA-MB-231 cells after incubation with FNDC5 for 48h observed by inverted microscope.

Observed MW

27 kDa.

PROPERTIES

Form

Lyophilized powder

Buffer

Reconstitute with 10mM PBS (pH7.4) to 0.1-1.0mg/ml. Do not vortex. Lyophilized from PBS (pH7.6), 5% Trehalose.

Preservative

No preservative

Storage

For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, store at -20ºC or below. After reconstitution, keep as concentrated solution. Avoid freeze-thaw cycles.

Region/Sequence

N-terminal His-Tag; Asp32~Glu143 (NP_001165411.2)

Expression System

HEK293 cells

Purity

> 97%

Endotoxin

< 1 EU/μg

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

fibronectin type III domain containing 5 , FRCP2 , irisin

Background

This gene encodes a secreted protein that is released from muscle cells during exercise. The encoded protein may participate in the development of brown fat. Translation of the precursor protein initiates at a non-AUG start codon at a position that is conserved as an AUG start codon in other organisms. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jun 2013]

Database

DATA IMAGES

Human FNDC5 protein, His tag (active). GTX00150-pro

GTX00150-pro Functional Assay Image

Cell apoptosis effect of GTX00150-pro Human FNDC5 protein (active).
(A) Unstimulated MDA-MB-231 cells cultured in DMEM for 48hrs.
(B) MDA-MB-231 cells cultured in DMEM, stimulated with 4 nM FNDC5 for 48hrs.

Human FNDC5 protein, His tag (active). GTX00150-pro

GTX00150-pro Functional Assay Image

The dose effect curve of GTX00150-pro Human FNDC5 protein (active) on MDA-MB-231 cells. After incubation of Human FNDC5 protein (active) for48 hrs. Cell viability was measured by Cell Counting Kit-8 (CCK-8).

Human FNDC5 protein, His tag (active). GTX00150-pro

GTX00150-pro Image

WB analysis of GTX00150-pro Human FNDC5 protein (active).

Human FNDC5 protein, His tag (active). GTX00150-pro

GTX00150-pro Image

SDS-PAGE analysis of GTX00150-pro Human FNDC5 protein (active).

REFERENCE

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REVIEW

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SDS
PBS.pdf
Package List Price ($)
$ 309