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Human FNDC5 protein (active)

Human FNDC5 protein (active). GTX00149-pro
Human FNDC5 protein (active). GTX00149-pro
Human FNDC5 protein (active). GTX00149-pro
Human FNDC5 protein (active). GTX00149-pro

Cat No. GTX00149-pro

Application Functional Assay
Species Human
Package
10 μg ($279)
APPLICATION

Application Note

Fibronectin type III domain-containing protein 5, the precursor of irisin, is a protein that is encoded by the FNDC5 gene. It was reported that FNDC5 significantly decreased cell number, migration and viability through apoptosis in malignant MDA-MB-231 cells. Thus MDA-MB-231 cells were seeded overnight at a density of 5000 cells/well, and treated with or without various concentrations of FNDC5 for 48h, then MDA-MB-231 cells were observed by inverted microscope and cell viability was measured by Cell Counting Kit-8 (CCK-8). Briefly, 10 μl of CCK-8 solution was added to each well of the plate, then measure the absorbance at 450nm using a microplate reader after incubating the plate for 1-4 hours at 37ºC. Cell apoptosis of MDA-MB-231 cells after incubation with FNDC5 for 48h observed by inverted microscope. FNDC5 significantly decreased cell viability of MDA-MB-231 cells, and the ED₅₀ for this effect is typically 1.72-2.70 nM.

Observed MW

18 kDa.
PROPERTIES

Form

Lyophilized powder

Buffer

Lyophilized from 20 mM Tris (pH 8.0) with 150 mM NaCl, 1 mM EDTA, 1 mM DTT, 0.01% SKL, 5% Trehalose, Proclin300. Reconstitute with 20 mM Tris and 150 mM NaCl (pH 8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Storage

For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, store at -20ºC or below. After reconstitution, keep as concentrated solution.Avoid freeze-thaw cycles.

Region/Sequence

N-terminal His-Tag; Leu2~Ile137 (NP_001165411.2)

Expression System

E. coli

Purity

> 90%

Endotoxin

< 1 EU/μg

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

fibronectin type III domain containing 5,FRCP2,irisin

Background

This gene encodes a secreted protein that is released from muscle cells during exercise. The encoded protein may participate in the development of brown fat. Translation of the precursor protein initiates at a non-AUG start codon at a position that is conserved as an AUG start codon in other organisms. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jun 2013]

Database

DATA IMAGES
Human FNDC5 protein (active). GTX00149-pro

GTX00149-pro Functional Assay Image

The dose effect curve of GTX00149-pro Human FNDC5 protein (active) on MDA-MB-231 cells. After incubation of Human FNDC5 protein (active) for 48 hrs, cell viability was measured by Cell Counting Kit-8 (CCK-8).

Human FNDC5 protein (active). GTX00149-pro

GTX00149-pro Functional Assay Image

Cell apoptosis effect of GTX00149-pro Human FNDC5 protein (active).
(A) Unstimulated MDA-MB-231 cells cultured in DMEM for 48hrs.
(B) MDA-MB-231 cells cultured in DMEM, stimulated with 4 nM FNDC5 for 48hrs.

Human FNDC5 protein (active). GTX00149-pro

GTX00149-pro Image

SDS-PAGE analysis of GTX00149-pro Human FNDC5 protein (active).

Human FNDC5 protein (active). GTX00149-pro

GTX00149-pro Image

WB analysis of GTX00149-pro Human FNDC5 protein (active).

REFERENCE
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REVIEW
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Package List Price ($)
$ 279