APPLICATION
Application Note
Complement Factor B (CFB) a component of the alternative pathway of complement activation. Upon activation of the alternative pathway, it is cleaved by complement factor D yielding the noncatalytic chain Ba and the catalytic subunit Bb. The active subunit Bb is a serine protease that associates with C3b to form the alternative pathway C3 convertase. The method of functional assay of CFB was tested in hemolysis assays. Two-fold dilute the recombinant human CFB with 0. 9% NaCl, 2 mmol/L MgCl2, and then add same volume of 1% rabbit erythrocyte (RaE) in 8 mmol/L EDTA, the negative control only without MgCl2. All the samples incubated at 37ºC. After 3 hours later, take 10 μl supernatant and 90μl TMB incubated at 37ºC for 5-10 minutes. Finally, add 50 μl stop solution to the wells and read at 450nm immediately. The minimal effective concentration of CFB is 0. 3125μg/ml.
Observed MW
44 kDa.
PROPERTIES
Form
Lyophilized powder
Buffer
Reconstitute with 20mM Tris and 150mM NaCl to 0.1-1.0mg/ml. Do not vortex. Lyophilized from 20mM Tris, 150mM NaCl, 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose.
Preservative
ProClin 300
Storage
For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, store at -20ºC or below. After reconstitution, keep as concentrated solution. Avoid freeze-thaw cycles.
Region/Sequence
N-terminal His and GST-Tag; Gly35~Asn160 (NP_001701.2)
Expression System
E. coli
Purity
> 90%
Endotoxin
< 1 EU/μg
Conjugation
Unconjugated
Note
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
Synonyms
complement factor B , AHUS4 , ARMD14 , BF , BFD , CFAB , CFBD , FB , FBI12 , GBG , H2-Bf , PBF2
Background
This gene encodes complement factor B, a component of the alternative pathway of complement activation. Factor B circulates in the blood as a single chain polypeptide. Upon activation of the alternative pathway, it is cleaved by complement factor D yielding the noncatalytic chain Ba and the catalytic subunit Bb. The active subunit Bb is a serine protease which associates with C3b to form the alternative pathway C3 convertase. Bb is involved in the proliferation of preactivated B lymphocytes, while Ba inhibits their proliferation. This gene localizes to the major histocompatibility complex (MHC) class III region on chromosome 6. This cluster includes several genes involved in regulation of the immune reaction. Polymorphisms in this gene are associated with a reduced risk of age-related macular degeneration. The polyadenylation site of this gene is 421 bp from the 5' end of the gene for complement component 2. [provided by RefSeq, Jul 2008]
Database
Research Area
DATA IMAGES
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GTX00269-pro Functional Assay Image
The hemagglutination effect of GTX00269-pro Human Factor B protein (active).
(A) 1% rabbit erythrocyte treated with 0.3125 μg/ml CFB, 8 mmol/l EDTA without MgCl₂.
(B) 1% rabbit erythrocyte treated with 0.3125 μg/ml CFB , 8 mmol/l EDTA with 0.5 mmol/l MgCl₂ for 1hr.
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GTX00269-pro Functional Assay Image
Hemolysis activity by various concentrations of GTX00269-pro Human Factor B protein (active).
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GTX00269-pro Image
WB analysis of GTX00269-pro Human Factor B protein (active).
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GTX00269-pro Image
SDS-PAGE analysis of GTX00269-pro Human Factor B protein (active).
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REFERENCE
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REVIEW
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