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Human Fibronectin protein (active)

Human Fibronectin protein (active). GTX00165-pro
Human Fibronectin protein (active). GTX00165-pro
Human Fibronectin protein (active). GTX00165-pro

Cat No. GTX00165-pro

Application

Functional Assay

Species

Human
Package
10 μg ($249)

APPLICATION

Application Note

Fibronectin (FN) is a high-molecular weight (~440kDa) glycoprotein of the extracellular matrix that binds to membrane-spanning receptor proteins called integrins. Fibronectins bind cell surfaces and various compounds including collagen, fibrin, heparin, DNA, and actin. Fibronectin has numerous functions. For example, it involved in cell adhesion, cell motility, opsonization, wound healing, maintenance of cell shape, and so on. Besides, Decorin (DCN) has been identified as an interactor of FN, thus a binding ELISA assay was conducted to detect the interaction of recombinant human FN and recombinant human DCN. Briefly, FN were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100 μl were then transferred to DCN-coated microtiter wells and incubated for 2h at 37ºC. Wells were washed with PBST and incubated for 1h with anti-FN pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37ºC. Finally, add 50 μl stop solution to the wells and read at 450nm immediately. The binding activity of of FN and DCN was in a dose dependent manner.

Observed MW

34 kDa.

PROPERTIES

Form

Lyophilized powder

Buffer

Lyophilized from 20 mM Tris (pH 8.0) with 150 mM NaCl, 1 mM EDTA, 1 mM DTT, 0.01% SKL, 5% Trehalose, Proclin300. Reconstitute with 20 mM Tris and 150 mM NaCl (pH 8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Storage

For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, store at -20ºC or below. After reconstitution, keep as concentrated solution.Avoid freeze-thaw cycles.

Region/Sequence

N-terminal His-Tag; Gly313~Ser607 (NP_001293058.1)

Expression System

E. coli

Purity

> 97%

Endotoxin

< 1 EU/μg

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

fibronectin 1 , CIG , ED-B , FINC , FN , FNZ , GFND , GFND2 , LETS , MSF , SMDCF

Background

This gene encodes fibronectin, a glycoprotein present in a soluble dimeric form in plasma, and in a dimeric or multimeric form at the cell surface and in extracellular matrix. The encoded preproprotein is proteolytically processed to generate the mature protein. Fibronectin is involved in cell adhesion and migration processes including embryogenesis, wound healing, blood coagulation, host defense, and metastasis. The gene has three regions subject to alternative splicing, with the potential to produce 20 different transcript variants, at least one of which encodes an isoform that undergoes proteolytic processing. The full-length nature of some variants has not been determined. [provided by RefSeq, Jan 2016]

Database

Research Area

DATA IMAGES

Human Fibronectin protein (active). GTX00165-pro

GTX00165-pro Functional Assay Image

Functional ELISA analysis of GTX00165-pro Human Fibronectin protein (active) which can bind immobilized DCN protein.

Human Fibronectin protein (active). GTX00165-pro

GTX00165-pro Image

WB analysis of GTX00165-pro Human Fibronectin protein (active).

Human Fibronectin protein (active). GTX00165-pro

GTX00165-pro Image

SDS-PAGE analysis of GTX00165-pro Human Fibronectin protein (active).

REFERENCE

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REVIEW

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Package List Price ($)
$ 249