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Human G-CSF protein (active)

Human G-CSF protein (active). GTX67010-pro

Cat No. GTX67010-pro

Application

Functional Assay

Species

Human
Package
50 μg ($289)

APPLICATION

Application Note

Measured in a cell proliferation assay using M-NFS-60 mouse myelogenous leukemia lymphoblast cells. The ED₅₀ for this effect is less than or equal to 0.3 ng/ml.

PROPERTIES

Form

Liquid

Buffer

PBS (pH 7.4) with 10% glycerol.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.5 mg/ml

Region/Sequence

Length: 175 a.a. Sequence: MTPLGPASSL PQSFLLKCLE QVRKIQGDGA ALQEKLCATY KLCHPEELVL LGHSLGIPWA PLSSCPSQAL QLAGCLSQLH SGLFLYQGLL QALEGISPEL GPTLDTLQLD VADFATTIWQ QMEELGMAPA LQPTQGAMPA FASAFQRRAG GVLVASHLQS FLEVSYRVLR HLAQP

Expression System

E.coli

Purification

purified by FPLC gel-filtration chromatography, after refolding of the isolated inclusion bodies in a renaturation buffer.

Purity

> 95% by SDS-PAGE.

Endotoxin

< 1.0 EU/μg (determined by LAL method)

Conjugation

Unconjugated

Note

For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

colony stimulating factor 3 , C17orf33 , CSF3OS , GCSF

Background

The protein encoded by this gene is a cytokine that controls the production, differentiation, and function of granulocytes. The active protein is found extracellularly. Alternatively spliced transcript variants have been described for this gene. [provided by RefSeq, May 2010]

Database

Research Area

DATA IMAGES

Human G-CSF protein (active). GTX67010-pro

GTX67010-pro Image

3 μg of GTX67010-pro Human G-CSF protein (active) by SDS-PAGE under reducing condition and visualized by coomassie blue stain

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Package List Price ($)
$ 289