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Human GDF8 / Myostatin protein, His tag

Human GDF8 / Myostatin protein, His tag. GTX00183-pro
Human GDF8 / Myostatin protein, His tag. GTX00183-pro
Human GDF8 / Myostatin protein, His tag. GTX00183-pro

Cat. No. GTX00183-pro

Application

 Functional Assay

Species

 Human
Package
10 μg ($289)
See all GDF8 / Myostatin protein products

APPLICATION

Application Note

Myostatin (MSTN) also known as growth differentiation factor 8(GDF-8) is a member of the TGF beta protein family. Myostatin is aa secreted growth differentiation factor that produced and released by myocytes. This protein negatively regulates skeletal muscle cell proliferation and differentiation. Besides, Bone Morphogenetic Protein 1 (BMP1) has been identified as an interactor of MSTN, thus a binding ELISA assay was conducted to detect the interaction of recombinant human MSTN and recombinant human BMP1. Briefly, MSTN were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100 μl were then transferred to BMP1-coated microtiter wells and incubated for 2h at 37ºC. Wells were washed with PBST and incubated for 1h with anti-MSTN pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37ºC. Finally, add 50 μl stop solution to the wells and read at 450nm immediately. The binding activity of of MSTN and BMP1 was in a dose dependent manner.

Observed MW

14 kDa.

PROPERTIES

Form

Lyophilized powder

Buffer

Reconstitute with 10mM PBS (pH7.4) to 0.1-1.0mg/ml. Do not vortex. Lyophilized from PBS (pH7.4).

Preservative

No preservative

Storage

For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, store at -20ºC or below. After reconstitution, keep as concentrated solution. Avoid freeze-thaw cycles.

Region/Sequence

N-terminal His-Tag; Asp267~Ser375 (NP_005250.1)

Expression System

E. coli

Purity

> 97%

Endotoxin

< 1 EU/μg

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

myostatin , GDF8 , MSLHP

Background

This gene encodes a secreted ligand of the TGF-beta (transforming growth factor-beta) superfamily of proteins. Ligands of this family bind various TGF-beta receptors leading to recruitment and activation of SMAD family transcription factors that regulate gene expression. The encoded preproprotein is proteolytically processed to generate each subunit of the disulfide-linked homodimer. This protein negatively regulates skeletal muscle cell proliferation and differentiation. Mutations in this gene are associated with increased skeletal muscle mass in humans and other mammals. [provided by RefSeq, Jul 2016]

Database

Research Area

DATA IMAGES

Human GDF8 / Myostatin protein, His tag. GTX00183-pro

GTX00183-pro Functional Assay Image

Functional ELISA analysis of GTX00183-pro Human GDF8 / Myostatin protein which can bind immobilized BMP1 protein.

Human GDF8 / Myostatin protein, His tag. GTX00183-pro

GTX00183-pro Image

WB analysis of GTX00183-pro Human GDF8 / Myostatin protein.

Human GDF8 / Myostatin protein, His tag. GTX00183-pro

GTX00183-pro Image

SDS-PAGE analysis of GTX00183-pro Human GDF8 / Myostatin protein.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Package List Price ($)
$ 289