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Human GDF9 protein, His tag

Human GDF9 protein, His tag. GTX00151-pro
Human GDF9 protein, His tag. GTX00151-pro
Human GDF9 protein, His tag. GTX00151-pro

Cat. No. GTX00151-pro

Application

 Functional Assay

Species

 Human
Package
10 μg ($349)
See all GDF9 protein products

APPLICATION

Application Note

GDF9 (Growth/differentiation factor 9) is an oocyte derived growth factora which belongs to the transforming growth factor-beta (TGFβ) superfamily. GDF9 is required for ovarian folliculogenesis and promotes primordial follicle development. S100A8 has been identified as an interactor of GDF9 through two-hybrid assay, thus a binding ELISA assay was conducted to detect the interaction of recombinant human GDF9 and recombinant human S100A8. Briefly, GDF9 were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100 μl were then transferred to S100A8-coated microtiter wells and incubated for 2h at 37ºC. Wells were washed with PBST and incubated for 1h with anti-GDF9 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37ºC. Finally, add 50 μl stop solution to the wells and read at 450nm immediately. The binding activity of of GDF9 and S100A8 was in a dose dependent manner.

Observed MW

18 kDa.

PROPERTIES

Form

Lyophilized powder

Buffer

Reconstitute with 20mM Tris and 150mM NaCl to 0.1-1.0mg/ml. Do not vortex. Lyophilized from 20mM Tris, 150mM NaCl, 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose.

Preservative

ProClin 300

Storage

For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, store at -20ºC or below. After reconstitution, keep as concentrated solution. Avoid freeze-thaw cycles.

Region/Sequence

N-terminal His-Tag; Gly320~Arg454 (NP_001275753.1)

Expression System

E. coli

Purity

> 90%

Endotoxin

< 1 EU/μg

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

growth differentiation factor 9 , POF14

Background

This gene encodes a secreted ligand of the TGF-beta (transforming growth factor-beta) superfamily of proteins. Ligands of this family bind various TGF-beta receptors leading to recruitment and activation of SMAD family transcription factors that regulate gene expression. The encoded preproprotein is proteolytically processed to generate each subunit of the disulfide-linked homodimer. This protein regulates ovarian function. Reduced expression of this gene may be associated with polycystic ovary syndrome and mutations in this gene may be more common in mothers of dizygotic twins. [provided by RefSeq, Jul 2016]

Database

Research Area

DATA IMAGES

Human GDF9 protein, His tag. GTX00151-pro

GTX00151-pro Functional Assay Image

Functional ELISA analysis of GTX00151-pro Human GDF9 protein which can bind immobilized S100A8 protein.

Human GDF9 protein, His tag. GTX00151-pro

GTX00151-pro Image

SDS-PAGE analysis of GTX00151-pro Human GDF9 protein.

Human GDF9 protein, His tag. GTX00151-pro

GTX00151-pro Image

WB analysis of GTX00151-pro Human GDF9 protein.

REFERENCE

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REVIEW

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SDS
Proclin.pdf
Package List Price ($)
$ 349