GeneTex

  • Country / Location Selection

United States (US)

Human GM-CSF protein, His tag (active)

Human GM-CSF protein, His tag (active). GTX00082-pro
Human GM-CSF protein, His tag (active). GTX00082-pro
Human GM-CSF protein, His tag (active). GTX00082-pro
Human GM-CSF protein, His tag (active). GTX00082-pro

Cat. No. GTX00082-pro

Reference 1

Application

 Functional Assay

Species

 Human
Package
10 μg ($289)
See all GM-CSF protein products

APPLICATION

Application Note

TF-1, the human erythroleukemia cell line, provides a good system for detecting the activity of GM-CSF for it is a cell line of immature erythroid origin that completely depends on interleukin 3 (IL-3) or granulocyte-macrophage colony-stimulating factor (GM-CSF) for long term growth. As reported, GM-CSF was also able to induce differentiation of human monoblastic leukemia cell line U937. TF-1 cells and U937 cells were incubated in the presence of various concentrations of rhGM-CSF, then cells were observed by inverted microscope. Cell proliferation of TF1 cells after incubation with GM-CSF (10 ng/ml) was for 3 days, and for U937 cells incubated with GM-CSF (10 ng/ml) was for 5 days.

Observed MW

19-26 kDa.

PROPERTIES

Form

Lyophilized powder

Buffer

Reconstitute with 20mM Tris and 150mM NaCl to 0.1-1.0mg/ml. Do not vortex. Lyophilized from 20mM Tris, 150mM NaCl, 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose.

Preservative

ProClin 300

Storage

For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, store at -20ºC or below. After reconstitution, keep as concentrated solution. Avoid freeze-thaw cycles.

Region/Sequence

N-terminal His-Tag; Ala18~Glu144 (NP_000749.2)

Expression System

HEK293 cells

Purity

> 95%

Endotoxin

< 1 EU/μg

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

colony stimulating factor 2 , CSF , GMCSF

Background

The protein encoded by this gene is a cytokine that controls the production, differentiation, and function of granulocytes and macrophages. The active form of the protein is found extracellularly as a homodimer. This gene has been localized to a cluster of related genes at chromosome region 5q31, which is known to be associated with interstitial deletions in the 5q- syndrome and acute myelogenous leukemia. Other genes in the cluster include those encoding interleukins 4, 5, and 13. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Human GM-CSF protein, His tag (active). GTX00082-pro

GTX00082-pro Functional Assay Image

Cell proliferation effect of GTX00082-pro Human GM-CSF protein (active).
(A) Unstimulated TF-1 cells cultured in RPMI-1640.
(B) TF-1 cells cultured in RPMI-1640, stimulated with GM-CSF (2 ng/ml).

Human GM-CSF protein, His tag (active). GTX00082-pro

GTX00082-pro Functional Assay Image

Cell differentiation effect of GTX00082-pro Human GM-CSF protein (active).
(A) Unstimulated U937 cells cultured in RPMI-1640.
(B) U937 cells cultured in RPMI-1640, stimulated with GM-CSF (10 ng/ml).

Human GM-CSF protein, His tag (active). GTX00082-pro

GTX00082-pro Image

SDS-PAGE analysis of GTX00082-pro Human GM-CSF protein (active).

Human GM-CSF protein, His tag (active). GTX00082-pro

GTX00082-pro Image

WB analysis of GTX00082-pro Human GM-CSF protein (active).

REFERENCE

Application Reference

Hashimoto M et al. Cell Mol Life Sci 2020; (Epub) Macrophage ubiquitin-specific protease 2 contributes to motility, hyperactivation, capacitation, and in vitro fertilization activity of mouse sperm.
Submit a Reference

REVIEW

There are currently no reviews for Human GM-CSF protein, His tag (active) (GTX00082-pro). Be the first to share your experience with this product.
Submit a Review
SDS
Proclin.pdf
Package List Price ($)
$ 289