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Human GM-CSF protein (active)

Human GM-CSF protein (active). GTX00082-pro
Human GM-CSF protein (active). GTX00082-pro
Human GM-CSF protein (active). GTX00082-pro
Human GM-CSF protein (active). GTX00082-pro

Cat No. GTX00082-pro

Application Functional Assay
Species Human
Package
10 μg ($269)
APPLICATION

Application Note

TF-1, the human erythroleukemia cell line, provides a good system for detecting the activity of GM-CSF for it is a cell line of immature erythroid origin that completely depends on interleukin 3 (IL-3) or granulocyte-macrophage colony-stimulating factor (GM-CSF) for long term growth. As reported, GM-CSF was also able to induce differentiation of human monoblastic leukemia cell line U937. TF-1 cells and U937 cells were incubated in the presence of various concentrations of rhGM-CSF, then cells were observed by inverted microscope. Cell proliferation of TF1 cells after incubation with GM-CSF (10 ng/ml) was for 3 days, and for U937 cells incubated with GM-CSF (10 ng/ml) was for 5 days.

Observed MW

19-26 kDa.
PROPERTIES

Form

Lyophilized powder

Buffer

Lyophilized from 20 mM Tris (pH 8.0) with 150 mM NaCl, 1 mM EDTA, 1 mM DTT, 0.01% SKL, 5% Trehalose, Proclin300. Reconstitute with 20 mM Tris and 150 mM NaCl (pH 8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Storage

For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, store at -20ºC or below. After reconstitution, keep as concentrated solution.Avoid freeze-thaw cycles.

Region/Sequence

N-terminal His-Tag; Ala18~Glu144 (NP_000749.2)

Expression System

293F cell

Purity

> 95%

Endotoxin

< 1 EU/μg

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

colony stimulating factor 2,CSF,GMCSF

Background

The protein encoded by this gene is a cytokine that controls the production, differentiation, and function of granulocytes and macrophages. The active form of the protein is found extracellularly as a homodimer. This gene has been localized to a cluster of related genes at chromosome region 5q31, which is known to be associated with interstitial deletions in the 5q- syndrome and acute myelogenous leukemia. Other genes in the cluster include those encoding interleukins 4, 5, and 13. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES
Human GM-CSF protein (active). GTX00082-pro

GTX00082-pro Functional Assay Image

Cell proliferation effect of GTX00082-pro Human GM-CSF protein (active).
(A) Unstimulated TF-1 cells cultured in RPMI-1640.
(B) TF-1 cells cultured in RPMI-1640, stimulated with GM-CSF (2 ng/ml).

Human GM-CSF protein (active). GTX00082-pro

GTX00082-pro Functional Assay Image

Cell differentiation effect of GTX00082-pro Human GM-CSF protein (active).
(A) Unstimulated U937 cells cultured in RPMI-1640.
(B) U937 cells cultured in RPMI-1640, stimulated with GM-CSF (10 ng/ml).

Human GM-CSF protein (active). GTX00082-pro

GTX00082-pro Image

WB analysis of GTX00082-pro Human GM-CSF protein (active).

Human GM-CSF protein (active). GTX00082-pro

GTX00082-pro Image

SDS-PAGE analysis of GTX00082-pro Human GM-CSF protein (active).

REFERENCE
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REVIEW
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Package List Price ($)
$ 269