PRODUCT
Summary
GM-CSF, a colony stimulating factor also known as CSF2, mediates the proliferation, differentiation, and functions of hematopoietic precursor cells. This bioactive recombinant human GM-CSF is a 14.6 kDa globular protein formed by a 128 a.a. polypeptide with two intramolecular disulfide bonds and two potential N-linked glycosylation sites.
APPLICATION
Application Note
The ED₅₀ was determined by a cell proliferation assay using TF-1 cells is ≤ 0.1 ng/ml, corresponding to a specific activity of ≥ 1 x 10⁷ units/mg.
Observed MW
14 kDa.
PROPERTIES
Form
Lyophilized powder
Buffer
Reconstitute with distilled water to 0.1 mg/ml. Lyophilized from 50mM PBS, 100mM NaCl, 5% Trehalose.
Preservative
No Preservative
Storage
Store at 4ºC or below. After reconstitution, keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. For long-term storage after reconstitution, aliquot and store at -70ºC or below. Do not vortex.
Region/Sequence
Human full-length GM-CSF protein (18-144 a.a. of NP_000749.2, signal peptide removed)
Expression System
E. Coli
Purity
>98%
Endotoxin
< 1 EU/μg
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
colony stimulating factor 2 , CSF , GMCSF
Cellular Localization
Secreted
Background
The protein encoded by this gene is a cytokine that controls the production, differentiation, and function of granulocytes and macrophages. The active form of the protein is found extracellularly as a homodimer. This gene has been localized to a cluster of related genes at chromosome region 5q31, which is known to be associated with interstitial deletions in the 5q- syndrome and acute myelogenous leukemia. Other genes in the cluster include those encoding interleukins 4, 5, and 13. [provided by RefSeq, Jul 2008]
Database
Research Area
DATA IMAGES
|
GTX137974-pro Functional Assay Image
Serial dilutions of Human GM-CSF protein (active) were added to TF-1 cells. Cell proliferation was measured and the linear portion of the curve was used to calculate the ED50.*This product has been tested against the competitor and the biological activity is better than competitor.
|
|
|
GTX137974-pro Image
1μg of GTX137974-pro Human GM-CSF protein (active) was analyzed using SDS-PAGE and stained with coomassie blue and captured by monochrome camera.
|
REFERENCE
There are currently no references for Human GM-CSF protein (active) (GTX137974-pro). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for Human GM-CSF protein (active) (GTX137974-pro). Be the first to share your experience with this product.
_FunctionalAssay_2_23081723_892.webp "Human GM-CSF protein (active). GTX137974-pro")
_Protein_2_23081723_903.webp "Human GM-CSF protein (active). GTX137974-pro")
_FunctionalAssay_2_23081723_892.webp "Human GM-CSF protein (active). GTX137974-pro")
_Protein_2_23081723_903.webp "Human GM-CSF protein (active). GTX137974-pro")