GeneTex
  • Country / Location Selection

United States (US)

Human GSTP1 protein, His tag (active)

Human GSTP1 protein, His tag (active). GTX67040-pro

Cat No. GTX67040-pro

Application

Functional Assay

Species

Human
Package
100 μg ($359)

APPLICATION

Application Note

Specific activity is > 80 units/mg, and is defined as the amount of enzyme that conjμgate 1.0 μmole of 1-chloro-2,4-dinitrobenzene (CDNB) with reduced glutathione per minute at pH 6.5 at 25ºC.

PROPERTIES

Form

Liquid

Buffer

20 mM Tris-HCl buffer (pH 7.0) with 30% glycerol, 1 mM EDTA, 0.1 mM PMSF.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.5 mg/ml

Region/Sequence

Full length protein, N-terminal His-Tag; Length: 246 a.a. Sequence: MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSMPPY TVVYFPVRGR CAALRMLLAD QGQSWKEEVV TVETWQEGSL KASCLYGQLP KFQDGDLTLY QSNTILRHLG RTLGLYGKDQ QEAALVDMVN DGVEDLRCKY ISLIYTNYEA GKDDYVKALP GQLKPFETLL SQNQGGKTFI VGDQISFADY NLLDLLLIHE VLAPGCLDAF PLLSAYVGRL SARPKLKAFL ASPEYVNLPI NGNGKQ

Expression System

E.coli

Purity

> 95% by SDS-PAGE.

Endotoxin

< 1.0 EU/μg (determined by LAL method)

Conjugation

Unconjugated

Note

For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

glutathione S-transferase pi 1 , DFN7 , FAEES3 , GST3 , GSTP , HEL-S-22 , PI

Background

Glutathione S-transferases (GSTs) are a family of enzymes that play an important role in detoxification by catalyzing the conjugation of many hydrophobic and electrophilic compounds with reduced glutathione. Based on their biochemical, immunologic, and structural properties, the soluble GSTs are categorized into 4 main classes: alpha, mu, pi, and theta. This GST family member is a polymorphic gene encoding active, functionally different GSTP1 variant proteins that are thought to function in xenobiotic metabolism and play a role in susceptibility to cancer, and other diseases. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Human GSTP1 protein, His tag (active). GTX67040-pro

GTX67040-pro Image

3 μg of GTX67040-pro Human GSTP1 protein (active) by SDS-PAGE under reducing condition and visualized by coomassie blue stain

REFERENCE

There are currently no references for Human GSTP1 protein, His tag (active) (GTX67040-pro). Be the first to share your publications with this product.

REVIEW

There are currently no reviews for Human GSTP1 protein, His tag (active) (GTX67040-pro). Be the first to share your experience with this product.
SDS
Glycerol.pdf
Package List Price ($)
$ 359