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Human GSTP1 protein (active)

Human GSTP1 protein (active). GTX00125-pro
Human GSTP1 protein (active). GTX00125-pro
Human GSTP1 protein (active). GTX00125-pro

Cat No. GTX00125-pro

Application Functional Assay
Species Human
Package
10 μg ($249)
APPLICATION

Application Note

GSTp (Glutathione S-transferase P) is an enzyme that plays an important role in detoxification by catalyzing the conjugation of many hydrophobic and electrophilic compounds with reduced glutathione. GSTP1 is identified as a CDK5 (Cyclin dependent kinase-5) regulatory protein, and is thought to regulates negatively CDK5 activity via p25/p35 translocation. Thus a binding ELISA assay was conducted to detect the interaction of recombinant human GSTp and recombinant human CDK5. Briefly, GSTp were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100 μl were then transferred to CDK5-coated microtiter wells and incubated for 2h at 37ºC. Wells were washed with PBST and incubated for 1h with anti-GSTp pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37ºC. Finally, add 50 μl stop solution to the wells and read at 450nm immediately. The binding activity of of GSTp and CDK5 was in a dose dependent manner.

Observed MW

25 kDa.
PROPERTIES

Form

Lyophilized powder

Buffer

Lyophilized from 20 mM Tris (pH 8.0) with 150 mM NaCl, 1 mM EDTA, 1 mM DTT, 0.01% SKL, 5% Trehalose, Proclin300. Reconstitute with 20 mM Tris and 150 mM NaCl (pH 8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Storage

For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, store at -20ºC or below. After reconstitution, keep as concentrated solution.Avoid freeze-thaw cycles.

Region/Sequence

Full length protein, N-terminal His-Tag; Met1~Gln210 (NP_000843.1)

Expression System

E. coli

Purity

> 95%

Endotoxin

< 1 EU/μg

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

glutathione S-transferase pi 1,DFN7,FAEES3,GST3,GSTP,HEL-S-22,PI

Background

Glutathione S-transferases (GSTs) are a family of enzymes that play an important role in detoxification by catalyzing the conjugation of many hydrophobic and electrophilic compounds with reduced glutathione. Based on their biochemical, immunologic, and structural properties, the soluble GSTs are categorized into 4 main classes: alpha, mu, pi, and theta. This GST family member is a polymorphic gene encoding active, functionally different GSTP1 variant proteins that are thought to function in xenobiotic metabolism and play a role in susceptibility to cancer, and other diseases. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES
Human GSTP1 protein (active). GTX00125-pro

GTX00125-pro Functional Assay Image

Functional ELISA analysis of GTX00125-pro Human GSTP1 protein (active) which can bind immobilized CDK5 protein.

Human GSTP1 protein (active). GTX00125-pro

GTX00125-pro Image

SDS-PAGE analysis of GTX00125-pro Human GSTP1 protein (active).

Human GSTP1 protein (active). GTX00125-pro

GTX00125-pro Image

WB analysis of GTX00125-pro Human GSTP1 protein (active).

REFERENCE
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REVIEW
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Package List Price ($)
$ 249