GeneTex

  • Country / Location Selection

United States (US)

Human Granzyme B protein, His tag (active)

Cat. No. GTX17622-pro

Application

 Functional Assay

Species

 Human
Package
5 μg ($219)
See all Human Granzyme B protein products
PRODUCT

Summary

GZMB produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 235 amino acids (19-247a.a.) and having a molecular mass of 26.5kDa. (Molecular size on SDS-PAGE will appear at approximately 28-40kDa). GZMB is expressed with an 6 amino acid His tag at C-Terminus and purified by proprietary chromatographic techniques.

APPLICATION

Application Note

Specific activity is > 7,000 pmol/min/ug, and is defined as the amount of enzyme that cleave 1pmole of Boc-Ala-Ala-Asp-SBzl at 37ºC.

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol, 1mM DTT

Preservative

No preservative

Storage

Store as concentrated solution. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, we recommend adding a carrier protein (0.1% HSA or BSA), then aliquot and store at -20ºC or below. Avoid freeze-thaw cycles.

Concentration

0.5 mg/ml (Please refer to the vial label for the specific concentration.)

Region/Sequence

C-terminal His-Tag; GEIIGGHEAK PHSRPYMAYL MIWDQKSLKR CGGFLIRDDF VLTAAHCWGS SINVTLGAHN IKEQEPTQQF IPVKRPIPHP AYNPKNFSND IMLLQLERKA KRTRAVQPLR LPSNKAQVKP GQTCSVAGWG QTAPLGKHSH TLQEVKMTVQ EDRKCESDLR HYYDSTIELC VGDPEIKKTS FKGDSGGPLV CNKVAQGIVS YGRNNGMPPR ACTKVSSFVH WIKKTMKRYH HHHHH

Expression System

Baculovirus (Sf9 cells)

Purity

≥ 95% by SDS-PAGE.

Conjugation

Unconjugated

Note

For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

granzyme B , C11 , CCPI , CGL-1 , CGL1 , CSP-B , CSPB , CTLA1 , CTSGL1 , HLP , SECT

Database

Research Area

REFERENCE

There are currently no references for Human Granzyme B protein, His tag (active) (GTX17622-pro). Be the first to share your publications with this product.
Submit a Reference

REVIEW

There are currently no reviews for Human Granzyme B protein, His tag (active) (GTX17622-pro). Be the first to share your experience with this product.
Submit a Review
SDS
PBS.pdf
Glycerol.pdf
Package List Price ($)
$ 219