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Human Granzyme M protein, His tag (active)

Human Granzyme M protein, His tag (active). GTX00112-pro
Human Granzyme M protein, His tag (active). GTX00112-pro
Human Granzyme M protein, His tag (active). GTX00112-pro

Cat. No. GTX00112-pro

Application

 Functional Assay

Species

 Human
Package
10 μg ($309)
See all Granzyme M protein products

APPLICATION

Application Note

GZMM (Granzyme M) is one of the neutral serine proteases, which is specifically expressed by NK cells and mediates a novel major and perforin-dependent cell death pathway. Granzyme M has been proven to targets α-Tubulin and disorganizes the microtuble network, besides, Ezrin has also been identified as a substrate of GZMM. Therefore, a catalytic assay was conducted to detect the protease activity of recombinant human GZMM using Hela cells lysates. Briefly, protein lysates were extracted from 2×107 Hela cells using Lysis Buffer, then incubated with normal or inactivated GZMM in 37ºC for 4h. Samples were immunoblotted using Abs β-actin as control, and Ezrin to detect the enzyme activity. And the recombinant human GZMM cleaved Ezrin.

Observed MW

26 kDa.

PROPERTIES

Form

Lyophilized powder

Buffer

Reconstitute with 20mM Tris and 150mM NaCl to 0.1-1.0mg/ml. Do not vortex. Lyophilized from 20mM Tris, 150mM NaCl, 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose.

Preservative

ProClin 300

Storage

For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, store at -20ºC or below. After reconstitution, keep as concentrated solution. Avoid freeze-thaw cycles.

Region/Sequence

N-terminal His-Tag; Ile26~Ala257 (NP_001245280.1)

Expression System

E. coli

Purity

> 90%

Endotoxin

< 1 EU/μg

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

granzyme M , LMET1 , MET1

Background

Human natural killer (NK) cells and activated lymphocytes express and store a distinct subset of neutral serine proteases together with proteoglycans and other immune effector molecules in large cytoplasmic granules. These serine proteases are collectively termed granzymes and include 4 distinct gene products: granzyme A, granzyme B, granzyme H, and the protein encoded by this gene, granzyme M. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Apr 2012]

Database

Research Area

DATA IMAGES

Human Granzyme M protein, His tag (active). GTX00112-pro

GTX00112-pro Functional Assay Image

Catalytic assay on HeLa cells treated by normal or inactivated GTX00112-pro Human Granzyme M protein (active) in 37ºC for 4hrs. And Human Granzyme M protein (active) cleaved Ezrin.
Recombinant protein concentrations: 4 μM

Human Granzyme M protein, His tag (active). GTX00112-pro

GTX00112-pro Image

WB analysis of GTX00112-pro Human Granzyme M protein (active).

Human Granzyme M protein, His tag (active). GTX00112-pro

GTX00112-pro Image

SDS-PAGE analysis of GTX00112-pro Human Granzyme M protein (active).

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SDS
Proclin.pdf
Package List Price ($)
$ 309