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Human Granzyme M protein (active)

Human Granzyme M protein (active). GTX00112-pro
Human Granzyme M protein (active). GTX00112-pro
Human Granzyme M protein (active). GTX00112-pro

Cat No. GTX00112-pro

Application Functional Assay
Species Human
Package
10 μg ($309)
APPLICATION

Application Note

GZMM (Granzyme M) is one of the neutral serine proteases, which is specifically expressed by NK cells and mediates a novel major and perforin-dependent cell death pathway. Granzyme M has been proven to targets α-Tubulin and disorganizes the microtuble network, besides, Ezrin has also been identified as a substrate of GZMM. Therefore, a catalytic assay was conducted to detect the protease activity of recombinant human GZMM using Hela cells lysates. Briefly, protein lysates were extracted from 2×107 Hela cells using Lysis Buffer, then incubated with normal or inactivated GZMM in 37ºC for 4h. Samples were immunoblotted using Abs β-actin as control, and Ezrin to detect the enzyme activity. And the recombinant human GZMM cleaved Ezrin.

Observed MW

26 kDa.
PROPERTIES

Form

Lyophilized powder

Buffer

Lyophilized from 20 mM Tris (pH 8.0) with 150 mM NaCl, 1 mM EDTA, 1 mM DTT, 0.01% SKL, 5% Trehalose, Proclin300. Reconstitute with 20 mM Tris and 150 mM NaCl (pH 8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Storage

For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, store at -20ºC or below. After reconstitution, keep as concentrated solution.Avoid freeze-thaw cycles.

Region/Sequence

N-terminal His-Tag; Ile26~Ala257 (NP_001245280.1)

Expression System

E. coli

Purity

> 90%

Endotoxin

< 1 EU/μg

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

granzyme M,LMET1,MET1

Background

Human natural killer (NK) cells and activated lymphocytes express and store a distinct subset of neutral serine proteases together with proteoglycans and other immune effector molecules in large cytoplasmic granules. These serine proteases are collectively termed granzymes and include 4 distinct gene products: granzyme A, granzyme B, granzyme H, and the protein encoded by this gene, granzyme M. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Apr 2012]

Database

Research Area

DATA IMAGES
Human Granzyme M protein (active). GTX00112-pro

GTX00112-pro Functional Assay Image

Catalytic assay on HeLa cells treated by normal or inactivated GTX00112-pro Human Granzyme M protein (active) in 37℃ for 4hrs. And Human Granzyme M protein (active) cleaved Ezrin.
Recombinant protein concentrations: 4 μM

Human Granzyme M protein (active). GTX00112-pro

GTX00112-pro Image

WB analysis of GTX00112-pro Human Granzyme M protein (active).

Human Granzyme M protein (active). GTX00112-pro

GTX00112-pro Image

SDS-PAGE analysis of GTX00112-pro Human Granzyme M protein (active).

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Package List Price ($)
$ 309