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Human Gremlin 1 protein (active)

Human Gremlin 1 protein (active). GTX00209-pro
Human Gremlin 1 protein (active). GTX00209-pro
Human Gremlin 1 protein (active). GTX00209-pro

Cat No. GTX00209-pro

Application Functional Assay
Species Human
Package
10 μg ($239)
APPLICATION

Application Note

Gremlin (GREM1) is an inhibitor in the TGF beta signaling pathway. GREM1 and other BMP antagonists are important in the survival of cancer stroma survival and proliferation in some cancers. The protein expression is found in many cancers and is thought to play important roles in uterine cervix, lung, ovary, kidney, breast, colon, pancreas, and sarcoma carcinomas. To test the effect of GREM1 on inhibit cell proliferation, 3T3 cells were seeded into triplicate wells of 96-well plates at a density of 5000 cells/well and allowed to attach, replaced with serum-free overnight, then the medium was replaced with 2% serum standard DMEM including various concentrations of recombinant human GREM1. After incubated for 96h,  cells were observed by inverted microscope and cell proliferation was measured by Cell Counting Kit-8 (CCK-8). Briefly, 10 μl of CCK-8 solution was added to each well of the plate, then the absorbance at 450nm was measured using a microplate reader after incubating the plate for 1-4 hours at 37ºC. Proliferation of 3T3 cells after incubation with GREM1 for 96h observed by inverted microscope. Cell viability was assessed by CCK-8 (Cell Counting Kit-8 ) assay after incubation with recombinant GREM1 for 96h that GREM1 significantly increased cell viability of 3T3 cells.

Observed MW

23 kDa.
PROPERTIES

Form

Lyophilized powder

Buffer

Lyophilized from 20 mM Tris (pH 8.0) with 150 mM NaCl, 1 mM EDTA, 1 mM DTT, 0.01% SKL, 5% Trehalose, Proclin300. Reconstitute with 20 mM Tris and 150 mM NaCl (pH 8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Storage

For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, store at -20ºC or below. After reconstitution, keep as concentrated solution.Avoid freeze-thaw cycles.

Region/Sequence

N-terminal His-Tag; Lys26~Asp184 (NP_001178252.1)

Expression System

E. coli

Purity

> 97%

Endotoxin

< 1 EU/μg

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

gremlin 1, DAN family BMP antagonist,C15DUPq,CKTSF1B1,CRAC1,CRCS4,DAND2,DRM,DUP15q,GREMLIN,HMPS,HMPS1,IHG-2,MPSH,PIG2

Background

This gene encodes a member of the BMP (bone morphogenic protein) antagonist family. Like BMPs, BMP antagonists contain cystine knots and typically form homo- and heterodimers. The CAN (cerberus and dan) subfamily of BMP antagonists, to which this gene belongs, is characterized by a C-terminal cystine knot with an eight-membered ring. The antagonistic effect of the secreted glycosylated protein encoded by this gene is likely due to its direct binding to BMP proteins. As an antagonist of BMP, this gene may play a role in regulating organogenesis, body patterning, and tissue differentiation. In mouse, this protein has been shown to relay the sonic hedgehog (SHH) signal from the polarizing region to the apical ectodermal ridge during limb bud outgrowth. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2010]

Database

Research Area

DATA IMAGES
Human Gremlin 1 protein (active). GTX00209-pro

GTX00209-pro Functional Assay Image

Cell suppression effect of GTX00209-pro Human Gremlin 1 protein (active). Cell viability was measured by Cell Counting Kit-8 (CCK-8).
(A) Unstimulated 3T3 cells cultured in DMEM for 96hrs.
(B) 3T3 cells cultured in DMEM, stimulated with 0.1 ng/ml GREM1 for 96hrs.

Human Gremlin 1 protein (active). GTX00209-pro

GTX00209-pro Image

SDS-PAGE analysis of GTX00209-pro Human Gremlin 1 protein (active).

Human Gremlin 1 protein (active). GTX00209-pro

GTX00209-pro Image

WB analysis of GTX00209-pro Human Gremlin 1 protein (active).

REFERENCE
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REVIEW
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Package List Price ($)
$ 239