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Human Grp78 protein, His tag (active)

Human Grp78 protein, His tag (active). GTX00434-pro

Cat. No. GTX00434-pro

Application

 Functional Assay

Species

 Human
Package
50 μg ($449)
See all Grp78 protein products

APPLICATION

Application Note

The protein has ATPase activity at the time of manufacture of 2.3 μM phosphate liberated/hr/μg protein in a 200 μl reaction at 37ºC (pH 8.0) in the presence of 20 μl of 1 mM ATP using a Malachite Green assay.

Observed MW

78 kDa.

PROPERTIES

Form

Liquid

Buffer

50mM Tris buffer, 150mM NaCl, 10% Glycerol

Preservative

No preservative

Storage

Store as concentrated solution. Aliquot and store at -20ºC or below. Avoid freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Region/Sequence

Full-length protein, His-Tag; NP_005338.1

Expression System

E. coli

Purification

Affinity Purified

Purity

> 90%

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

heat shock protein family A (Hsp70) member 5 , BIP , GRP78 , HEL-S-89n , MIF2

Background

The protein encoded by this gene is a member of the heat shock protein 70 (HSP70) family. It is localized in the lumen of the endoplasmic reticulum (ER), and is involved in the folding and assembly of proteins in the ER. As this protein interacts with many ER proteins, it may play a key role in monitoring protein transport through the cell.[provided by RefSeq, Sep 2010]

Database

Research Area

DATA IMAGES

Human Grp78 protein, His tag (active). GTX00434-pro

GTX00434-pro Image

SDS-PAGE analysis of GTX00434-pro Human Grp78 protein (active).

REFERENCE

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SDS
Glycerol.pdf
Package List Price ($)
$ 449