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Human Guanylate kinase protein, His tag (active)

Human Guanylate kinase protein, His tag (active). GTX67045-pro

Cat No. GTX67045-pro

Application

Functional Assay

Species

Human
Package
10 μg ($199)

APPLICATION

Application Note

Specific activity is > 100 units/mg and is defined as the amount of enzyme that convert 1.0 μmole of GMP and ATP to GDP and ADP per minute at pH 7.5 at 37ºC in coupled system with PK/LDH.

PROPERTIES

Form

Liquid

Buffer

20 mM Tris-HCl buffer (pH 8.0) with 100 mM NaCl, 1 mM DTT, 10% glycerol.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml

Region/Sequence

Full length protein, N-terminal His-Tag; Length: 217 a.a. Sequence: MGSSHHHHHH SSGLVPRGSH MSGPRPVVLS GPSGAGKSTL LKRLLQEHSG IFGFSVSHTT RNPRPGEENG KDYYFVTREV MQRDIAAGDF IEHAEFSGNL YGTSKVAVQA VQAMNRICVL DVDLQGVRNI KATDLRPIYI SVQPPSLHVL EQRLRQRNTE TEESLVKRLA AAQADMESSK EPGLFDVVII NDSLDQAYAE LKEALSEEIK KAQRTGA

Expression System

E.coli

Purity

> 90% by SDS-PAGE.

Conjugation

Unconjugated

Note

For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

guanylate kinase 1 , GMK

Background

The protein encoded by this gene is an enzyme that catalyzes the transfer of a phosphate group from ATP to guanosine monophosphate (GMP) to form guanosine diphosphate (GDP). The encoded protein is thought to be a good target for cancer chemotherapy. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jun 2011]

Database

DATA IMAGES

Human Guanylate kinase protein, His tag (active). GTX67045-pro

GTX67045-pro Image

3 μg of GTX67045-pro Human Guanylate kinase protein (active) by SDS-PAGE under reducing condition and visualized by coomassie blue stain

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Package List Price ($)
$ 199