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Human HGF protein (active)

Human HGF protein (active). GTX00206-pro
Human HGF protein (active). GTX00206-pro
Human HGF protein (active). GTX00206-pro
Human HGF protein (active). GTX00206-pro

Cat No. GTX00206-pro

Application Functional Assay
Species Human
Package
10 μg ($259)
APPLICATION

Application Note

Hepatocyte growth factor (HGF) is a paracrine cellular growth, motility and morphogenic factor. It is secreted by mesenchymal cells and targets and acts primarily upon epithelial cells and endothelial cells, but also acts on haemopoietic progenitor cells and T cells. It has been shown to have a major role in embryonic organ development, specifically in myogenesis, in adult organ regeneration, and in wound healing. Besides, Heparan sulfate proteogly (HSPG) has been identified as an interactor of HGF, thus a binding ELISA assay was conducted to detect the interaction of recombinant human HGF and recombinant human HSPG. Briefly, HGF were diluted serially in PBS, with 0. 01% BSA (pH 7. 4). Duplicate samples of 100 μl were then transferred to HSPG -coated microtiter wells and incubated for 2h at 37ºC. Wells were washed with PBST and incubated for 1h with anti- HGF pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37ºC. Finally, add 50 μl stop solution to the wells and read at 450nm immediately. The binding activity of HGF and HSPG was in a dose dependent manner.
To test the effect of HGF on cell proliferation of HepG2 cell line, cells were seeded into triplicate wells of 96-well plates at a density of 2000 cells/well and allowed to attach overnight, then the medium was replaced with serum-free standard DMEM prior to the addition of various concentrations of HGF. After incubated for 72h, cells were observed by inverted microscope and cell proliferation was measured by Cell Counting Kit-8 (CCK-8). Briefly, 10 μl of CCK-8 solution was added to each well of the plate, then measure the absorbance at 450nm using a microplate reader after incubating the plate for 1-4 hours at 37ºC.

Observed MW

30 kDa.
PROPERTIES

Form

Lyophilized powder

Buffer

Lyophilized from 20 mM Tris (pH 8.0) with 150 mM NaCl, 1 mM EDTA, 1 mM DTT, 0.01% SKL, 5% Trehalose, Proclin300. Reconstitute with 20 mM Tris and 150 mM NaCl (pH 8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Storage

For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, store at -20ºC or below. After reconstitution, keep as concentrated solution.Avoid freeze-thaw cycles.

Region/Sequence

N-terminal His-Tag; Val495~Ser728 (NP_000592.3)

Expression System

E. coli

Purity

> 90%

Endotoxin

< 1 EU/μg

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

hepatocyte growth factor,DFNB39,F-TCF,HGFB,HPTA,SF

Background

This gene encodes a protein that binds to the hepatocyte growth factor receptor to regulate cell growth, cell motility and morphogenesis in numerous cell and tissue types. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed to generate alpha and beta chains, which form the mature heterodimer. This protein is secreted by mesenchymal cells and acts as a multi-functional cytokine on cells of mainly epithelial origin. This protein also plays a role in angiogenesis, tumorogenesis, and tissue regeneration. Although the encoded protein is a member of the peptidase S1 family of serine proteases, it lacks peptidase activity. Mutations in this gene are associated with nonsyndromic hearing loss. [provided by RefSeq, Nov 2015]

Database

Research Area

DATA IMAGES
Human HGF protein (active). GTX00206-pro

GTX00206-pro Functional Assay Image

Cell proliferation effect of GTX00206-pro Human HGF protein (active). Cell viability was measured by Cell Counting Kit-8 (CCK-8).
(A) Unstimulated HepG2 cells cultured in DMEM for 72hrs.
(B) HepG2 cells cultured in DMEM, stimulated with 0.01 ng/ml HGF for 72hrs.

Human HGF protein (active). GTX00206-pro

GTX00206-pro Functional Assay Image

Functional ELISA analysis of GTX00206-pro Human HGF protein (active) which can bind immobilized HSPG protein.

Human HGF protein (active). GTX00206-pro

GTX00206-pro Image

WB analysis of GTX00206-pro Human HGF protein (active).

Human HGF protein (active). GTX00206-pro

GTX00206-pro Image

SDS-PAGE analysis of GTX00206-pro Human HGF protein (active).

REFERENCE
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Package List Price ($)
$ 259