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Human HMGB1 protein (active)

Human HMGB1 protein (active). GTX00154-pro
Human HMGB1 protein (active). GTX00154-pro
Human HMGB1 protein (active). GTX00154-pro

Cat No. GTX00154-pro

Application Functional Assay
Species Human
Package
10 μg ($329)
APPLICATION

Application Note

High Mobility Group Protein 1 (HMG1), also known as high mobility group box 1 proteinbelongs to high mobility group and contains HMG-box domain. HMG1 is one of the most important chromatin proteins. This nuclear protein organizes the DNA and regulates transcription. It supports transcription of many genes in interactions with many transcription factors. HMGB1 is secreted by immune cells (like macrophages, monocytes and dendritic cells) through leaderless secretory pathway. Activated macrophages and monocytes secrete HMGB1 as a cytokine mediator of Inflammation. Besides, Tumor Protein p53 (TP53) has been identified as an interactor of HMG1, thus a binding ELISA assay was conducted to detect the interaction of recombinant human HMG1 and recombinant human TP53. Briefly, HMG1 were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100 μl were then transferred to TP53-coated microtiter wells and incubated for 2h at 37ºC. Wells were washed with PBST and incubated for 1h with anti-HMG1 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37ºC. Finally, add 50 μl stop solution to the wells and read at 450nm immediately. The binding activity of HMG1 and TP53 was in a dose dependent manner.

Observed MW

57 kDa.
PROPERTIES

Form

Lyophilized powder

Buffer

Lyophilized from PBS (pH 7.4) with 0.01% SKL, 1 mM DTT, 5% Trehalose, Proclin300. Reconstitute with 10 mM PBS (pH 7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex

Storage

For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, store at -20ºC or below. After reconstitution, keep as concentrated solution.Avoid freeze-thaw cycles.

Region/Sequence

Full length protein, N-terminal His-Tag; Met1~Glu215 (NP_001300821.1)

Expression System

293F cell

Purity

> 95%

Endotoxin

< 1 EU/μg

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

high mobility group box 1,HMG-1,HMG1,HMG3,SBP-1

Background

This gene encodes a protein that belongs to the High Mobility Group-box superfamily. The encoded non-histone, nuclear DNA-binding protein regulates transcription, and is involved in organization of DNA. This protein plays a role in several cellular processes, including inflammation, cell differentiation and tumor cell migration. Multiple pseudogenes of this gene have been identified. Alternative splicing results in multiple transcript variants that encode the same protein. [provided by RefSeq, Sep 2015]

Database

Research Area

DATA IMAGES
Human HMGB1 protein (active). GTX00154-pro

GTX00154-pro Functional Assay Image

Functional ELISA analysis of GTX00154-pro Human HMGB1 protein (active) which can bind immobilized TP53 protein.

Human HMGB1 protein (active). GTX00154-pro

GTX00154-pro Image

WB analysis of GTX00154-pro Human HMGB1 protein (active).

Human HMGB1 protein (active). GTX00154-pro

GTX00154-pro Image

SDS-PAGE analysis of GTX00154-pro Human HMGB1 protein (active).

REFERENCE
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REVIEW
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Package List Price ($)
$ 329