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Human HNMT protein, His tag (active)

Human HNMT protein, His tag (active). GTX67050-pro

Cat. No. GTX67050-pro

Application

 Functional Assay

Species

 Human
Package
5 μg ($219)
See all HNMT protein products

APPLICATION

Application Note

Specific activity is > 200 nmol/min/mg, and is defined as the amount of enzyme that transfer 1.0 nmole of methyl group per minute at 37ºC.

PROPERTIES

Form

Liquid

Buffer

20mM Tris-HCl, 10% Glycerol

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Region/Sequence

Full length protein, N-terminal His-Tag; Length: 328 a.a. Sequence: MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSMASS MRSLFSDHGK YVESFRRFLN HSTEHQCMQE FMDKKLPGII GRIGDTKSEI KILSIGGGAG EIDLQILSKV QAQYPGVCIN NEVVEPSAEQ IAKYKELVAK TSNLENVKFA WHKETSSEYQ SRMLEKKELQ KWDFIHMIQM LYYVKDIPAT LKFFHSLLGT NAKMLIIVVS GSSGWDKLWK KYGSRFPQDD LCQYITSDDL TQMLDNLGLK YECYDLLSTM DISDCFIDGD ENGDLLWDFL TETCNFNATA PPDLRAELGK DLQEPEFSAK KEGKVLFNNT LSFIVIEA

Expression System

E. coli

Purity

> 90% by SDS-PAGE.

Conjugation

Unconjugated

Note

For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

histamine N-methyltransferase , HMT , HNMT-S1 , HNMT-S2 , MRT51

Background

In mammals, histamine is metabolized by two major pathways: N(tau)-methylation via histamine N-methyltransferase and oxidative deamination via diamine oxidase. This gene encodes the first enzyme which is found in the cytosol and uses S-adenosyl-L-methionine as the methyl donor. In the mammalian brain, the neurotransmitter activity of histamine is controlled by N(tau)-methylation as diamine oxidase is not found in the central nervous system. A common genetic polymorphism affects the activity levels of this gene product in red blood cells. Multiple alternatively spliced transcript variants that encode different proteins have been found for this gene. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Human HNMT protein, His tag (active). GTX67050-pro

GTX67050-pro Image

3 μg of GTX67050-pro Human HNMT protein (active) by SDS-PAGE under reducing condition and visualized by coomassie blue stain

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SDS
Glycerol.pdf
Package List Price ($)
$ 219