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Human Hsp70 protein, His tag (active)

Human Hsp70 protein, His tag (active). GTX00446-pro

Cat. No. GTX00446-pro

Application

Functional Assay

Species

Human
Package
50 μg ($539)

APPLICATION

Application Note

The protein has ATPase activity at the time of manufacture of 3.3 μM phosphate liberated/hr/μg protein in a 200 μl reaction at 37ºC (pH 7.5) in the presence of 20 μl of 1 mM ATP using a Malachite Green assay.

Observed MW

70 kDa.

PROPERTIES

Form

Liquid

Buffer

20mM Na-Phosphate (pH7.5), 150mM NaCl, 10% Glycerol, 200mM Imidazole

Preservative

No preservative

Storage

Store as concentrated solution. Aliquot and store at -20ºC or below. Avoid freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Region/Sequence

Full-length protein, His-Tag; NP_005336.3

Expression System

E. coli

Purification

Affinity Purified

Purity

> 90%

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

heat shock protein family A (Hsp70) member 1A , HEL-S-103 , HSP70-1 , HSP70-1A , HSP70-2 , HSP70.1 , HSP70.2 , HSP70I , HSP72 , HSPA1

Background

This intronless gene encodes a 70kDa heat shock protein which is a member of the heat shock protein 70 family. In conjuction with other heat shock proteins, this protein stabilizes existing proteins against aggregation and mediates the folding of newly translated proteins in the cytosol and in organelles. It is also involved in the ubiquitin-proteasome pathway through interaction with the AU-rich element RNA-binding protein 1. The gene is located in the major histocompatibility complex class III region, in a cluster with two closely related genes which encode similar proteins. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Human Hsp70 protein, His tag (active). GTX00446-pro

GTX00446-pro Image

SDS-PAGE analysis of GTX00446-pro Human Hsp70 protein (active).

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Package List Price ($)
$ 539