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Human IGF1 protein, His tag (active)

Human IGF1 protein, His tag (active). GTX00085-pro
Human IGF1 protein, His tag (active). GTX00085-pro
Human IGF1 protein, His tag (active). GTX00085-pro
Human IGF1 protein, His tag (active). GTX00085-pro

Cat. No. GTX00085-pro

Application

 Functional Assay

Species

 Human
Package
10 μg ($209)
See all IGF1 protein products

APPLICATION

Application Note

insulin-like growth factor I (IGF1), is a hormone similar in molecular structure to insulin but have a much higher growth-promoting activity, it belongs to a family of proteins involved in mediating growth and development. It is reported that IGF1 induces the proliferation, migration, differentiation of a large types of cells including the MCF-7 breast cancer cell line. To test the effect of growth factors on proliferation,  MCF-7 cells were seeded into triplicate wells of 96-well plates at a density of 2000 cells/well and allowed to attach overnight, then the medium was replaced with serum-free standard DMEM  prior to the addition of various concentrations of IGF-1. After incubated for 72h,  cells were observed by inverted microscope and cell proliferation was measured by Cell Counting Kit-8 (CCK-8). Briefly, 10 μl of CCK-8 solution was added to each well of the plate, then measure the absorbance at 450nm using a microplate reader after incubating the plate for 1-4 hours at 37ºC. Cell proliferation of MCF-7 cells after incubation with IGF1 for 72h observed by inverted microscope.

Observed MW

14 kDa.

PROPERTIES

Form

Lyophilized powder

Buffer

Reconstitute with 20mM Tris and 150mM NaCl to 0.1-1.0mg/ml. Do not vortex. Lyophilized from 20mM Tris, 150mM NaCl, 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose.

Preservative

ProClin 300

Storage

For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, store at -20ºC or below. After reconstitution, keep as concentrated solution. Avoid freeze-thaw cycles.

Region/Sequence

N-terminal His-Tag; EFKLAAALEGGRKLEF+Gly49~Ala118 (NP_000609.1)

Expression System

HEK293 cells

Purity

> 95%

Endotoxin

< 1 EU/μg

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

insulin like growth factor 1 , IGF , IGF-I , IGFI , MGF

Background

The protein encoded by this gene is similar to insulin in function and structure and is a member of a family of proteins involved in mediating growth and development. The encoded protein is processed from a precursor, bound by a specific receptor, and secreted. Defects in this gene are a cause of insulin-like growth factor I deficiency. Alternative splicing results in multiple transcript variants encoding different isoforms that may undergo similar processing to generate mature protein. [provided by RefSeq, Sep 2015]

Database

Research Area

DATA IMAGES

Human IGF1 protein, His tag (active). GTX00085-pro

GTX00085-pro Functional Assay Image

The dose effect curve of GTX00085-pro Human IGF1 protein (active) on MCF-7 cells. After incubation of Human IGF1 protein (active) for 72 hrs, cell proliferation was measured by Cell Counting Kit-8 (CCK-8).

Human IGF1 protein, His tag (active). GTX00085-pro

GTX00085-pro Functional Assay Image

Cell proliferation effect of GTX00085-pro Human IGF1 protein (active).
(A) Unstimulated MCF-7 cells cultured in serum-free DMEM for 72hrs.
(B) MCF-7 cells cultured in serum-free DMEM, stimulated with 10 ng/ml IGF1 for 72hrs.

Human IGF1 protein, His tag (active). GTX00085-pro

GTX00085-pro Image

SDS-PAGE analysis of GTX00085-pro Human IGF1 protein (active).

Human IGF1 protein, His tag (active). GTX00085-pro

GTX00085-pro Image

WB analysis of GTX00085-pro Human IGF1 protein (active).

REFERENCE

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REVIEW

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SDS
Proclin.pdf
Package List Price ($)
$ 209