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Human IGF1 protein (active)

Human IGF1 protein (active). GTX00250-pro
Human IGF1 protein (active). GTX00250-pro
Human IGF1 protein (active). GTX00250-pro
Human IGF1 protein (active). GTX00250-pro

Cat No. GTX00250-pro

Application Functional Assay
Species Human
Package
10 μg ($169)
APPLICATION

Application Note

insulin-like growth factor 1 (IGF1), also called somatomedin C is a hormone similar in molecular structure to insulin. It plays an important role in childhood growth and continues to have anabolic effects in adults. A synthetic analog of IGF1, mecasermin, is used for the treatment of growth failure. IGF1 consists of 70 amino acids in a single chain with three intramolecular disulfide bridges. IGF1 has a molecular weight of 7, 649 Daltons. Besides, insulin Like Growth Factor Binding Protein 4 (IGFBP4) has been identified as an interactor of IGF1, thus a binding ELISA assay was conducted to detect the interaction of recombinant human IGF1 and recombinant human IGFBP4. Briefly, IGF1 were diluted serially in PBS, with 0. 01% BSA (pH 7. 4). Duplicate samples of 100 μl were then transferred to IGFBP4-coated microtiter wells and incubated for 2h at 37ºC. Wells were washed with PBST and incubated for 1h with anti-VDR pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37ºC. Finally, add 50 μl stop solution to the wells and read at 450nm immediately. The binding activity of IGF1 and IGFBP4 was in a dose dependent manner.
To measure the effect of IGF1 on cell proliferation, breast cancer MCF-7 cells were seeded into triplicate wells of 96-well plates at a density of 5000 cells/well and allowed to attach, replaced with serum-free overnight, then the medium was replaced with 1% serum standard DMEM  prior to the addition of various concentrations of recombinant human IGF1. After incubated for 96h,  cells were observed by inverted microscope and cell proliferation was measured by Cell Counting Kit-8 (CCK-8). Briefly, 10 μl of CCK-8 solution was added to each well of the plate, then the absorbance at 450nm was measured using a microplate reader after incubating the plate for 1-4 hours at 37ºC. Proliferation of MCF-7 cells after incubation with IGF1 for 72h observed by inverted microscop. Cell viability was assessed by CCK-8 assay after incubation with recombinant IGF1 for 72h, and IGF1 significantly increased cell viability of MCF-7 cells.

Observed MW

40 kDa.
PROPERTIES

Form

Lyophilized powder

Buffer

Lyophilized from 20 mM Tris (pH 8.0) with 150 mM NaCl, 1 mM EDTA, 1 mM DTT, 0.01% SKL, 5% Trehalose, Proclin300. Reconstitute with 20 mM Tris and 150 mM NaCl (pH 8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Storage

For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, store at -20ºC or below. After reconstitution, keep as concentrated solution.Avoid freeze-thaw cycles.

Region/Sequence

N-terminal His-Tag; Gly49~Ala118 (NP_000609.1)

Expression System

E. coli

Purity

> 95%

Endotoxin

< 1 EU/μg

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

insulin like growth factor 1 , IGF , IGF-I , IGFI , MGF

Background

The protein encoded by this gene is similar to insulin in function and structure and is a member of a family of proteins involved in mediating growth and development. The encoded protein is processed from a precursor, bound by a specific receptor, and secreted. Defects in this gene are a cause of insulin-like growth factor I deficiency. Alternative splicing results in multiple transcript variants encoding different isoforms that may undergo similar processing to generate mature protein. [provided by RefSeq, Sep 2015]

Database

Research Area

DATA IMAGES
Human IGF1 protein (active). GTX00250-pro

GTX00250-pro Functional Assay Image

Functional ELISA analysis of GTX00250-pro Human IGF1 protein (active) which can bind immobilized IGFBP4 protein.

Human IGF1 protein (active). GTX00250-pro

GTX00250-pro Functional Assay Image

Cell proliferation effect of GTX00250-pro Human IGF1 protein (active). Cell viability was measured by Cell Counting Kit-8 (CCK-8).
(A) Unstimulated MCF-7 cells cultured in DMEM for 72hrs.
(B) MCF-7 cells cultured in DMEM, stimulated with 10 ng/ml IGF1 for 72hrs.

Human IGF1 protein (active). GTX00250-pro

GTX00250-pro Image

WB analysis of GTX00250-pro Human IGF1 protein (active).

Human IGF1 protein (active). GTX00250-pro

GTX00250-pro Image

SDS-PAGE analysis of GTX00250-pro Human IGF1 protein (active).

REFERENCE
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REVIEW
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Package List Price ($)
$ 169