APPLICATION
Application Note
IL1R1 (Interleukin 1 Receptor Type I), also known as CD121a, is an important mediator involved in many cytokine induced immune and inflammatory responses. It belongs to the interleukin-1 receptor family, and is a receptor for interleukin 1 alpha (IL1a), interleukin 1 beta (IL1B), and interleukin 1 receptor antagonist (IL1RA). Thus, a binding ELISA assay was conducted to detect the association of IL1R1 with IL1a. Briefly, IL1R1 were diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100 μl were then transferred to IL1a-coated microtiter wells and incubated for 2h at 37ºC. Wells were washed with PBST and incubated for 1h with anti-IL1R1 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37ºC. Finally, add 50 μl stop solution to the wells and read at 450nm immediately. The binding activity of IL1R1 with IL1a was in a dose dependent manner.
Observed MW
14 kDa.
PROPERTIES
Form
Lyophilized powder
Buffer
Reconstitute with 20mM Tris and 150mM NaCl to 0.1-1.0mg/ml. Do not vortex. Lyophilized from 20mM Tris, 150mM NaCl, 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose.
Preservative
ProClin 300
Storage
For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, store at -20ºC or below. After reconstitution, keep as concentrated solution. Avoid freeze-thaw cycles.
Region/Sequence
N-terminal His-Tag; Asp21~Ile109 (NP_000868.1)
Expression System
E. coli
Purity
> 95%
Endotoxin
< 1 EU/μg
Conjugation
Unconjugated
Note
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
Synonyms
interleukin 1 receptor type 1 , CD121A , D2S1473 , IL-1R-alpha , IL1R , IL1RA , P80
Background
This gene encodes a cytokine receptor that belongs to the interleukin-1 receptor family. The encoded protein is a receptor for interleukin-1 alpha, interleukin-1 beta, and interleukin-1 receptor antagonist. It is an important mediator involved in many cytokine-induced immune and inflammatory responses. This gene is located in a cluster of related cytokine receptor genes on chromosome 2q12. [provided by RefSeq, Dec 2013]
Database
Research Area
DATA IMAGES
|
GTX00091-pro Functional Assay Image
Functional ELISA analysis of GTX00091-pro Human IL1 Receptor 1 protein which can bind immobilized IL1 alpha protein.
|
|
|
GTX00091-pro Image
SDS-PAGE analysis of GTX00091-pro Human IL1 Receptor 1 protein.
|
|
|
GTX00091-pro Image
WB analysis of GTX00091-pro Human IL1 Receptor 1 protein.
|
REFERENCE
There are currently no references for Human IL1 Receptor 1 protein, His tag (GTX00091-pro). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for Human IL1 Receptor 1 protein, His tag (GTX00091-pro). Be the first to share your experience with this product.
