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Human IL1 Receptor 1 protein (active)

Human IL1 Receptor 1 protein (active). GTX00091-pro
Human IL1 Receptor 1 protein (active). GTX00091-pro
Human IL1 Receptor 1 protein (active). GTX00091-pro

Cat No. GTX00091-pro

Application

Functional Assay

Species

Human
Package
10 μg ($239)

APPLICATION

Application Note

IL1R1 (Interleukin 1 Receptor Type I), also known as CD121a, is an important mediator involved in many cytokine induced immune and inflammatory responses. It belongs to the interleukin-1 receptor family, and is a receptor for interleukin 1 alpha (IL1a), interleukin 1 beta (IL1B), and interleukin 1 receptor antagonist (IL1RA). Thus, a binding ELISA assay was conducted to detect the association of IL1R1 with IL1a. Briefly, IL1R1 were diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100 μl were then transferred to IL1a-coated microtiter wells and incubated for 2h at 37ºC. Wells were washed with PBST and incubated for 1h with anti-IL1R1 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37ºC. Finally, add 50 μl stop solution to the wells and read at 450nm immediately. The binding activity of IL1R1 with IL1a was in a dose dependent manner.

Observed MW

14 kDa.

PROPERTIES

Form

Lyophilized powder

Buffer

Lyophilized from 20 mM Tris (pH 8.0) with 150 mM NaCl, 1 mM EDTA, 1 mM DTT, 0.01% SKL, 5% Trehalose, Proclin300. Reconstitute with 20 mM Tris and 150 mM NaCl (pH 8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Storage

For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, store at -20ºC or below. After reconstitution, keep as concentrated solution.Avoid freeze-thaw cycles.

Region/Sequence

N-terminal His-Tag; Asp21~Ile109 (NP_000868.1)

Expression System

E. coli

Purity

> 95%

Endotoxin

< 1 EU/μg

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

interleukin 1 receptor type 1 , CD121A , D2S1473 , IL-1R-alpha , IL1R , IL1RA , P80

Background

This gene encodes a cytokine receptor that belongs to the interleukin-1 receptor family. The encoded protein is a receptor for interleukin-1 alpha, interleukin-1 beta, and interleukin-1 receptor antagonist. It is an important mediator involved in many cytokine-induced immune and inflammatory responses. This gene is located in a cluster of related cytokine receptor genes on chromosome 2q12. [provided by RefSeq, Dec 2013]

Database

Research Area

DATA IMAGES

Human IL1 Receptor 1 protein (active). GTX00091-pro

GTX00091-pro Functional Assay Image

Functional ELISA analysis of GTX00091-pro Human IL1 Receptor 1 protein (active) which can bind immobilized IL1 alpha protein.

Human IL1 Receptor 1 protein (active). GTX00091-pro

GTX00091-pro Image

SDS-PAGE analysis of GTX00091-pro Human IL1 Receptor 1 protein (active).

Human IL1 Receptor 1 protein (active). GTX00091-pro

GTX00091-pro Image

WB analysis of GTX00091-pro Human IL1 Receptor 1 protein (active).

REFERENCE

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REVIEW

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