APPLICATION
Application Note
IL1RA (interleukin-1 receptor antagonist) is an agent that binds to the cell surface interleukin-1 receptor (IL-1R), which would prevent IL-1 from intracellular signal transduction. Thus, a binding ELISA assay was constructed to detect the association of recombinant human IL1RA with recombinant human IL1R1. Briefly, IL1RA were diluted serially in PBS with 0.1% BSA (pH 7.4). Duplicate samples of 100 μl were then transferred to IL1R1-coated microtiter wells and incubated for 2h at 37ºC. Wells were washed with PBST and incubated for 1h with anti-IL1RA pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37ºC. Finally, add 50 μl stop solution to the wells and read at 450nm immediately. The binding activity of IL1RA with IL1R1 was in a dose dependent manner.
Observed MW
18 kDa.
PROPERTIES
Form
Lyophilized powder
Buffer
Reconstitute with 20mM Tris and 150mM NaCl to 0.1-1.0mg/ml. Do not vortex. Lyophilized from 20mM Tris, 150mM NaCl, 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose.
Preservative
ProClin 300
Storage
For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, store at -20ºC or below. After reconstitution, keep as concentrated solution. Avoid freeze-thaw cycles.
Region/Sequence
N-terminal His-Tag; Arg26~Glu177 (NP_000568.1)
Expression System
E. coli
Purity
> 95%
Endotoxin
< 1 EU/μg
Conjugation
Unconjugated
Note
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
Synonyms
interleukin 1 receptor antagonist , DIRA , ICIL-1RA , IL-1RN , IL-1ra , IL-1ra3 , IL1F3 , IL1RA , IRAP , MVCD4
Background
The protein encoded by this gene is a member of the interleukin 1 cytokine family. This protein inhibits the activities of interleukin 1, alpha (IL1A) and interleukin 1, beta (IL1B), and modulates a variety of interleukin 1 related immune and inflammatory responses. This gene and five other closely related cytokine genes form a gene cluster spanning approximately 400 kb on chromosome 2. A polymorphism of this gene is reported to be associated with increased risk of osteoporotic fractures and gastric cancer. Several alternatively spliced transcript variants encoding distinct isoforms have been reported. [provided by RefSeq, Jan 2016]
Database
Research Area
DATA IMAGES
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GTX00108-pro Functional Assay Image
Functional ELISA analysis of GTX00108-pro Human IL1 Receptor antagonist protein which can bind immobilized IL1R1 protein.
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GTX00108-pro Image
WB analysis of GTX00108-pro Human IL1 Receptor antagonist protein.
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GTX00108-pro Image
SDS-PAGE analysis of GTX00108-pro Human IL1 Receptor antagonist protein.
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REFERENCE
There are currently no references for Human IL1 Receptor antagonist protein, His tag (GTX00108-pro). Be the first to share your publications with this product.
REVIEW
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