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Human IL1 Receptor antagonist protein (active)

Human IL1 Receptor antagonist protein (active). GTX00108-pro
Human IL1 Receptor antagonist protein (active). GTX00108-pro
Human IL1 Receptor antagonist protein (active). GTX00108-pro

Cat No. GTX00108-pro

Application Functional Assay
Species Human
Package
10 μg ($279)
APPLICATION

Application Note

IL1RA (interleukin-1 receptor antagonist) is an agent that binds to the cell surface interleukin-1 receptor (IL-1R), which would prevent IL-1 from intracellular signal transduction. Thus, a binding ELISA assay was constructed to detect the association of recombinant human IL1RA with recombinant human IL1R1. Briefly, IL1RA were diluted serially in PBS with 0.1% BSA (pH 7.4). Duplicate samples of 100 μl were then transferred to IL1R1-coated microtiter wells and incubated for 2h at 37ºC. Wells were washed with PBST and incubated for 1h with anti-IL1RA pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37ºC. Finally, add 50 μl stop solution to the wells and read at 450nm immediately. The binding activity of IL1RA with IL1R1 was in a dose dependent manner.

Observed MW

18 kDa.
PROPERTIES

Form

Lyophilized powder

Buffer

Lyophilized from 20 mM Tris (pH 8.0) with 150 mM NaCl, 1 mM EDTA, 1 mM DTT, 0.01% SKL, 5% Trehalose, Proclin300. Reconstitute with 20 mM Tris and 150 mM NaCl (pH 8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Storage

For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, store at -20ºC or below. After reconstitution, keep as concentrated solution.Avoid freeze-thaw cycles.

Region/Sequence

N-terminal His-Tag; Arg26~Glu177 (NP_000568.1)

Expression System

E. coli

Purity

> 95%

Endotoxin

< 1 EU/μg

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

interleukin 1 receptor antagonist,DIRA,ICIL-1RA,IL-1RN,IL-1ra,IL-1ra3,IL1F3,IL1RA,IRAP,MVCD4

Background

The protein encoded by this gene is a member of the interleukin 1 cytokine family. This protein inhibits the activities of interleukin 1, alpha (IL1A) and interleukin 1, beta (IL1B), and modulates a variety of interleukin 1 related immune and inflammatory responses. This gene and five other closely related cytokine genes form a gene cluster spanning approximately 400 kb on chromosome 2. A polymorphism of this gene is reported to be associated with increased risk of osteoporotic fractures and gastric cancer. Several alternatively spliced transcript variants encoding distinct isoforms have been reported. [provided by RefSeq, Jan 2016]

Database

Research Area

DATA IMAGES
Human IL1 Receptor antagonist protein (active). GTX00108-pro

GTX00108-pro Functional Assay Image

Functional ELISA analysis of GTX00108-pro Human IL1 Receptor antagonist protein (active) which can bind immobilized IL1R1 protein.

Human IL1 Receptor antagonist protein (active). GTX00108-pro

GTX00108-pro Image

WB analysis of GTX00108-pro Human IL1 Receptor antagonist protein (active).

Human IL1 Receptor antagonist protein (active). GTX00108-pro

GTX00108-pro Image

SDS-PAGE analysis of GTX00108-pro Human IL1 Receptor antagonist protein (active).

REFERENCE
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REVIEW
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Package List Price ($)
$ 279