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Human IL1 alpha protein (active)

Human IL1 alpha protein (active). GTX00067-pro
Human IL1 alpha protein (active). GTX00067-pro
Human IL1 alpha protein (active). GTX00067-pro
Human IL1 alpha protein (active). GTX00067-pro

Cat No. GTX00067-pro

Application Functional Assay
Species Human
Package
10 μg ($309)
APPLICATION

Application Note

IL1α (Interleukin-1 alpha) is a member of the interleukin 1 cytokine family. This cytokine is produced by monocytes and macrophages as a proprotein, which is proteolytically processed and released in response to cell injury, and thus induces cell apoptosis. It is reported that exposure of MCF-7 cells to certain concentration of IL1α results in inhibition of cell growth. Thus, an cell proliferation assay of MCF-7 was conducted with the addition of IL1α. MCF-7 cells were seeded overnight at a density of 5000 cells/well, and then treated with or without various concentrations of IL1α for 72h, then cells were observed by inverted microscope and cell viability was measured by Cell Counting Kit-8 (CCK-8). Briefly, 10 μl of CCK-8 solution was added to each well of the plate, then measure the absorbance at 450nm using a microplate reader after incubating the plate for 1-4 hours in at 37ºC. Inhibition of MCF-7 cell proliferation after incubation with IL1α for 72h observed by inverted microscope.

Observed MW

21 kDa.
PROPERTIES

Form

Lyophilized powder

Buffer

Lyophilized from 20 mM Tris (pH 8.0) with 150 mM NaCl, 1 mM EDTA, 1 mM DTT, 0.01% SKL, 5% Trehalose, Proclin300. Reconstitute with 20 mM Tris and 150 mM NaCl (pH 8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Storage

For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, store at -20ºC or below. After reconstitution, keep as concentrated solution.Avoid freeze-thaw cycles.

Region/Sequence

N-terminal His-Tag; Ser113~Ala271 (NP_000566.3)

Expression System

E. coli

Purity

> 97%

Endotoxin

< 1 EU/μg

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

interleukin 1 alpha,IL-1A,IL1,IL1-ALPHA,IL1F1

Background

The protein encoded by this gene is a member of the interleukin 1 cytokine family. This cytokine is a pleiotropic cytokine involved in various immune responses, inflammatory processes, and hematopoiesis. This cytokine is produced by monocytes and macrophages as a proprotein, which is proteolytically processed and released in response to cell injury, and thus induces apoptosis. This gene and eight other interleukin 1 family genes form a cytokine gene cluster on chromosome 2. It has been suggested that the polymorphism of these genes is associated with rheumatoid arthritis and Alzheimer's disease. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES
Human IL1 alpha protein (active). GTX00067-pro

GTX00067-pro Functional Assay Image

Cell suppression effect of GTX00067-pro Human IL1 alpha protein (active).
(A) Unstimulated MCF-7 cells cultured in DMEM for 72hrs.
(B) MCF-7 cells cultured in DMEM, stimulated with 1 ng/ml IL1α for 72hrs.

Human IL1 alpha protein (active). GTX00067-pro

GTX00067-pro Functional Assay Image

The inhibitory effect of GTX00067-pro Human IL1 alpha protein (active) on the cell proliferation of MCF-7 cells. After incubation of Human IL1 alpha protein (active) for 72hrs, cell viability was measured by Cell Counting Kit-8 (CCK-8).

Human IL1 alpha protein (active). GTX00067-pro

GTX00067-pro Image

WB analysis of GTX00067-pro Human IL1 alpha protein (active).

Human IL1 alpha protein (active). GTX00067-pro

GTX00067-pro Image

SDS-PAGE analysis of GTX00067-pro Human IL1 alpha protein (active).

REFERENCE
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REVIEW
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