APPLICATION
Application Note
IL1α (Interleukin-1 alpha) is a member of the interleukin 1 cytokine family. This cytokine is produced by monocytes and macrophages as a proprotein, which is proteolytically processed and released in response to cell injury, and thus induces cell apoptosis. It is reported that exposure of MCF-7 cells to certain concentration of IL1α results in inhibition of cell growth. Thus, an cell proliferation assay of MCF-7 was conducted with the addition of IL1α. MCF-7 cells were seeded overnight at a density of 5000 cells/well, and then treated with or without various concentrations of IL1α for 72h, then cells were observed by inverted microscope and cell viability was measured by Cell Counting Kit-8 (CCK-8). Briefly, 10 μl of CCK-8 solution was added to each well of the plate, then measure the absorbance at 450nm using a microplate reader after incubating the plate for 1-4 hours in at 37ºC. Inhibition of MCF-7 cell proliferation after incubation with IL1α for 72h observed by inverted microscope.
Observed MW
21 kDa.
PROPERTIES
Form
Lyophilized powder
Buffer
Reconstitute with 20mM Tris and 150mM NaCl to 0.1-1.0mg/ml. Do not vortex. Lyophilized from 20mM Tris, 150mM NaCl, 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose.
Preservative
ProClin 300
Storage
For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, store at -20ºC or below. After reconstitution, keep as concentrated solution. Avoid freeze-thaw cycles.
Region/Sequence
N-terminal His-Tag; Ser113~Ala271 (NP_000566.3)
Expression System
E. coli
Purity
> 97%
Endotoxin
< 1 EU/μg
Conjugation
Unconjugated
Note
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
Synonyms
interleukin 1 alpha , IL-1A , IL1 , IL1-ALPHA , IL1F1
Background
The protein encoded by this gene is a member of the interleukin 1 cytokine family. This cytokine is a pleiotropic cytokine involved in various immune responses, inflammatory processes, and hematopoiesis. This cytokine is produced by monocytes and macrophages as a proprotein, which is proteolytically processed and released in response to cell injury, and thus induces apoptosis. This gene and eight other interleukin 1 family genes form a cytokine gene cluster on chromosome 2. It has been suggested that the polymorphism of these genes is associated with rheumatoid arthritis and Alzheimer's disease. [provided by RefSeq, Jul 2008]
Database
Research Area
DATA IMAGES
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GTX00067-pro Functional Assay Image
The inhibitory effect of GTX00067-pro Human IL1 alpha protein (active) on the cell proliferation of MCF-7 cells. After incubation of Human IL1 alpha protein (active) for 72hrs, cell viability was measured by Cell Counting Kit-8 (CCK-8).
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GTX00067-pro Functional Assay Image
Cell suppression effect of GTX00067-pro Human IL1 alpha protein (active).
(A) Unstimulated MCF-7 cells cultured in DMEM for 72hrs.
(B) MCF-7 cells cultured in DMEM, stimulated with 1 ng/ml IL1α for 72hrs.
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GTX00067-pro Image
WB analysis of GTX00067-pro Human IL1 alpha protein (active).
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GTX00067-pro Image
SDS-PAGE analysis of GTX00067-pro Human IL1 alpha protein (active).
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REFERENCE
There are currently no references for Human IL1 alpha protein, His tag (active) (GTX00067-pro). Be the first to share your publications with this product.
REVIEW
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